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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2014.03.019
|2 doi
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|a pubmed25n0798.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Guan, Xin
|e verfasserin
|4 aut
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|a Actin marker lines in grapevine reveal a gatekeeper function of guard cells
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 30.03.2015
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2014 Elsevier GmbH. All rights reserved.
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|a Resistance to abiotic and biotic stress is a central topic for sustainable agriculture, especially in grapevine, one of the field crops with the highest economic output per acreage. As early cellular factors for plant defense, actin microfilaments (AF) are of high relevance. We therefore generated a transgenic actin marker line for grapevine by expressing a fusion protein between green fluorescent protein and the second actin-binding domain of Arabidopsis (Arabidopsis thaliana) fimbrin, AtFIM1. Based on this first cytoskeletal-marker line in grapevine, the response of AFs to phytopathogenic microorganisms could be followed in vivo. Upon inoculation with fluorescently labeled strains of phytopathogenic bacteria, actin responses were confined to the guard cells. In contrast, upon contact with zoospores of Plasmopara viticola, not only the guard cells, but also epidermal pavement cells, where no zoospores had attached responded with the formation of a perinuclear actin basket. Our data support the hypothesis that guard cells act as pacemakers of defense, dominating the responses of the remaining epidermal cells
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Actin
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|a Gram-negative bacteria
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|a Guard cell
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|a Plasmopara viticola
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|a Vitis vinifera ‘Chardonnay’, transgenic
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|a Actins
|2 NLM
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| 650 |
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|a Genetic Markers
|2 NLM
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|a Buchholz, Günther
|e verfasserin
|4 aut
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|a Nick, Peter
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 171(2014), 13 vom: 15. Aug., Seite 1164-73
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:171
|g year:2014
|g number:13
|g day:15
|g month:08
|g pages:1164-73
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|u http://dx.doi.org/10.1016/j.jplph.2014.03.019
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