When fathers are instant losers : homogenization of rDNA loci in recently formed Cardamine × schulzii trigenomic allopolyploid

© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 203(2014), 4 vom: 10. Sept., Seite 1096-1108
1. Verfasser: Zozomová-Lihová, Judita (VerfasserIn)
Weitere Verfasser: Mandáková, Terezie, Kovaříková, Alena, Mühlhausen, Andreas, Mummenhoff, Klaus, Lysak, Martin A, Kovařík, Aleš
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Brassicaceae concerted evolution hybridization hybrids polyploidy rDNA DNA, Ribosomal DNA, Ribosomal Spacer
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520 |a Recently formed allopolyploids represent an excellent system to study the impacts of hybridization and genomic duplication on genome structure and evolution. Here we explored the 35SrRNA genes (rDNA) in the Cardamine × schulzii allohexaploid that was formed by two subsequent hybridization events within the past c. 150 yr. The rDNA loci were analyzed by cloning, next generation sequencing (NGS), RT-PCR and FISH methods. The primary C. × insueta triploid hybrid derived from C. rivularis (♀) and C. amara (♂) had gene ratios highly skewed towards maternal sequences. Similarly, C. × schulzii, originating from the secondary hybridization event involving C. × insueta (♀) and C. pratensis (♂), showed a reduction in paternal rDNA homeologs despite an excess of chromosomes inherited from C. pratensis. We also identified novel rDNA loci in C. × schulzii, suggesting that lost loci might be slowly reinstalled by translocation (but not recombination) of genes from partner genomes. Prevalent clonal propagation of allopolyploids, C. × insueta and C. × schulzii, indicates that concerted evolution of rDNA may occur in the absence of extensive meiotic cycles. Adoption of NGS in rDNA variant analysis is highly informative for deciphering the evolutionary histories of allopolyploid species with ongoing homogenization processes 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Brassicaceae 
650 4 |a concerted evolution 
650 4 |a hybridization 
650 4 |a hybrids 
650 4 |a polyploidy 
650 4 |a rDNA 
650 7 |a DNA, Ribosomal  |2 NLM 
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700 1 |a Mandáková, Terezie  |e verfasserin  |4 aut 
700 1 |a Kovaříková, Alena  |e verfasserin  |4 aut 
700 1 |a Mühlhausen, Andreas  |e verfasserin  |4 aut 
700 1 |a Mummenhoff, Klaus  |e verfasserin  |4 aut 
700 1 |a Lysak, Martin A  |e verfasserin  |4 aut 
700 1 |a Kovařík, Aleš  |e verfasserin  |4 aut 
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