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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1111/nph.12870
|2 doi
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|a pubmed24n0796.xml
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|a (DE-627)NLM238812537
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|a (NLM)24890496
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Sueldo, Daniela
|e verfasserin
|4 aut
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|a Dynamic hydrolase activities precede hypersensitive tissue collapse in tomato seedlings
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 30.03.2015
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2014 The Authors New Phytologist © 2014 New Phytologist Trust.
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|a Hydrolases such as subtilases, vacuolar processing enzymes (VPEs) and the proteasome play important roles during plant programmed cell death (PCD). We investigated hydrolase activities during PCD using activity-based protein profiling (ABPP), which displays the active proteome using probes that react covalently with the active site of proteins. We employed tomato (Solanum lycopersicum) seedlings undergoing synchronized hypersensitive cell death by co-expressing the avirulence protein Avr4 from Cladosporium fulvum and the tomato resistance protein Cf-4. Cell death is blocked in seedlings grown at high temperature and humidity, and is synchronously induced by decreasing temperature and humidity. ABPP revealed that VPEs and the proteasome are not differentially active, but that activities of papain-like cysteine proteases and serine hydrolases, including Hsr203 and P69B, increase before hypersensitive tissue collapse, whereas the activity of a carboxypeptidase-like enzyme is reduced. Similar dynamics were observed for these enzymes in the apoplast of tomato challenged with C. fulvum. Unexpectedly, these challenged plants also displayed novel isoforms of secreted putative VPEs. In the absence of tissue collapse at high humidity, the hydrolase activity profile is already altered completely, demonstrating that changes in hydrolase activities precede hypersensitive tissue collapse
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Hsr203
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|a P69
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|a activity-based protein profiling (ABPP)
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|a papain-like cysteine protease
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|a programmed cell death (PCD)
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|a tomato (Solanum lycopersicum)
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4 |
|a vacuolar processing enzyme (VPE)
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| 650 |
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|a Serine Proteases
|2 NLM
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| 650 |
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|a EC 3.4.-
|2 NLM
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| 700 |
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|a Ahmed, Ali
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Misas-Villamil, Johana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Colby, Tom
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tameling, Wladimir
|e verfasserin
|4 aut
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| 700 |
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|a Joosten, Matthieu H A J
|e verfasserin
|4 aut
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| 700 |
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|a van der Hoorn, Renier A L
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 203(2014), 3 vom: 02. Aug., Seite 913-25
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:203
|g year:2014
|g number:3
|g day:02
|g month:08
|g pages:913-25
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|u http://dx.doi.org/10.1111/nph.12870
|3 Volltext
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|j 2014
|e 3
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|h 913-25
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