Activation of MKK9-MPK3/MPK6 enhances phosphate acquisition in Arabidopsis thaliana

Activation of MKK9-MPK3/MPK6 enhances phosphate acquisition in Arabidopsis thaliana

© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 203(2014), 4 vom: 17. Sept., Seite 1146-1160
1. Verfasser: Lei, Lei (VerfasserIn)
Weitere Verfasser: Li, Yuan, Wang, Qian, Xu, Juan, Chen, Yifang, Yang, Hailian, Ren, Dongtao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana MKK9 MPK3 MPK6 Pi responses phosphate signal transduction pathway Arabidopsis Proteins mehr... Mutant Proteins Phosphates AtMPK3 protein, Arabidopsis EC 2.7.11.24 MPK6 protein, Arabidopsis Mitogen-Activated Protein Kinases MKK9 protein, Arabidopsis EC 2.7.12.2 Mitogen-Activated Protein Kinase Kinases
Beschreibung
Zusammenfassung:© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.
Despite the abundance of phosphorus in soil, very little is available as phosphate (Pi) for plants. Plants often experience low Pi (LP) stress. Intensive studies have been conducted to reveal the mechanism used by plants to deal with LP; however, Pi sensing and signal transduction pathways are not fully understood. Using in-gel kinase assays, we determined the activities of MPK3 and MPK6 in Arabidopsis thaliana seedlings under both LP and Pi-sufficient (Murashige and Skoog, MS) conditions. Using MKK9 mutant transgenic and crossed mutants, we analyzed the functions of MPK3 and MPK6 in regulating Pi responses of seedlings. The regulation of Pi responses by downstream components of MKK9-MPK3/MPK6 was also screened. LP treatment activated MPK3 and MPK6. Under both LP and MS conditions, mpk3 and mpk6 seedlings took up and accumulated less Pi than the wild-type; activation of MKK9-MPK3/MPK6 in transgenic seedlings induced the transcription of Pi acquisition-related genes and enhanced Pi uptake and accumulation, whereas its activation suppressed the transcription of anthocyanin biosynthetic genes and anthocyanin accumulation; WRKY75 was downstream of MKK9-MPK3/MPK6 when regulating the accumulation of Pi and anthocyanin, and the transcription of Pi acquisition-related and anthocyanin biosynthetic genes. These results suggest that the MKK9-MPK3/MPK6 cascade is part of the Pi signaling pathway in plants
Beschreibung:Date Completed 03.04.2015
Date Revised 10.04.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.12872