|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM238548333 |
| 003 |
DE-627 |
| 005 |
20231224114031.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/eru211
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0795.xml
|
| 035 |
|
|
|a (DE-627)NLM238548333
|
| 035 |
|
|
|a (NLM)24860187
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Xiong, Li
|e verfasserin
|4 aut
|
| 245 |
1 |
4 |
|a The non-catalytic N-terminal domain of ACS7 is involved in the post-translational regulation of this gene in Arabidopsis
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 01.04.2015
|
| 500 |
|
|
|a Date Revised 29.07.2014
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
|
| 520 |
|
|
|a Post-transcriptional control of the expression of the 1-aminocyclopropane-1-carboxylate synthase (ACS) gene family is important for maintaining appropriate levels of ethylene production. However, the molecular mechanism underlying the post-transcriptional regulation of type 3 ACS proteins remains unclear. Multiple sequence alignment revealed that the N-terminus of type 3 ACSs was longer than that of other ACSs. Fusing the N-terminal 54 residues of ACS7, the sole type 3 ACS in Arabidopsis, to the β-glucuronidase (GUS) reporter significantly decreased the stability of N(7(1-54))-GUS fusion protein. Among these 54 residues, residues 1-14 conferred this negative effect on the GUS fusion gene. Consistently, a truncated form of ACS7 lacking residues 1-14 was more stable than full-length ACS7 when transgenically expressed in Arabidopsis and led to a more severe ethylene response phenotype in the light-grown transgenic seedlings. Interestingly, the ACS7 N-terminus had no effect on the stability of N(7)-GUS and ACS7 proteins at the etiolated seedling stage. Both exogenous 1-aminocyclopropane-1-carboxylic acid (ACC) treatment and salt stress could rescue the levels of accumulation of N(7)-GUS fusion protein in light-grown seedlings. These results suggest that the non-catalytic N-terminus of ACS7 is involved in its own post-translational regulation. The proteasome inhibitor MG132 suppressed degradation of full-length ACS7 in vivo, but had little effect on the N-terminal truncated form of ACS7, indicating that the N-terminus mediates the regulation of ACS7 stability through the ubiquitin-26S proteasome pathway
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a 1-Aminocyclopropane-1-carboxylate synthase 7
|
| 650 |
|
4 |
|a Arabidopsis
|
| 650 |
|
4 |
|a N-terminus
|
| 650 |
|
4 |
|a ethylene
|
| 650 |
|
4 |
|a post-translational regulation
|
| 650 |
|
4 |
|a protein stability.
|
| 650 |
|
7 |
|a Proteasome Endopeptidase Complex
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.25.1
|2 NLM
|
| 650 |
|
7 |
|a ATP dependent 26S protease
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.99.-
|2 NLM
|
| 650 |
|
7 |
|a Lyases
|2 NLM
|
| 650 |
|
7 |
|a EC 4.-
|2 NLM
|
| 650 |
|
7 |
|a 1-aminocyclopropanecarboxylate synthase
|2 NLM
|
| 650 |
|
7 |
|a EC 4.4.1.14
|2 NLM
|
| 700 |
1 |
|
|a Xiao, Dong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xu, Xinxin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Guo, Zhaoxia
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Ning Ning
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 15 vom: 15. Aug., Seite 4397-408
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:65
|g year:2014
|g number:15
|g day:15
|g month:08
|g pages:4397-408
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/eru211
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 65
|j 2014
|e 15
|b 15
|c 08
|h 4397-408
|