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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1021/la501381v
|2 doi
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|a pubmed24n0794.xml
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|a (NLM)24835108
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Ma, Wan-Fu
|e verfasserin
|4 aut
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|a Magnetic MSPZrO₂ microspheres with yolk-shell structure
|b designed synthesis and application in highly selective enrichment of phosphopeptides
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.04.2015
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|a Date Revised 10.06.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Magnetic yolk-shell MSPZrO2 microspheres consisting of a movable magnetic supraparticle (MSP) core and a crystalline ZrO2 shell were synthesized via a two-step controlled "sol-gel" approach for the first time. First, a large amount of the generated hydrolyzate Zr(OH)4 was firmly fixed onto the surface of the cross-linked polymethylacrylic acid matrix via a strong hydrogen-bonding interaction between Zr(OH)4 and the carboxyl groups. Then a calcination process was adopted to convert the Zr(OH)4 into a continuous ZrO2 shell and simultaneously make the ZrO2 shell crystallized. At the same time, the polymer matrix could be selectively removed to form a yolk-shell structure, which has better dispersibility and higher adsorbing efficiency of phosphopeptides than its solid counterpart. The formation mechanism of such yolk-shell microspheres could be reasonably proved by the results of TEM, TGA, VSM, XRD, and FT-IR characterization. By taking advantage of the unique properties, the yolk-shell MSP@ZrO2 exhibited high specificity and great capability in selective enrichment of phosphopeptides, and a total of 33 unique phosphopeptides mapped to 33 different phosphoproteins had been identified from 1 mL of human saliva. This result clearly demonstrated that the yolk-shell MSP@ZrO2 has great performance in purifying and identifying the low-abundant phosphopeptides from real complex biological samples. Moreover, the synthetic method can be used to produce hybrid yolk-shell MSP@ZrO2-TiO2
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Phosphopeptides
|2 NLM
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|a Zhang, Cheng
|e verfasserin
|4 aut
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|a Zhang, Yu-Ting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Meng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Jia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Ying
|e verfasserin
|4 aut
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| 700 |
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|a Lu, Hao-Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Chang-Chun
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 22 vom: 10. Juni, Seite 6602-11
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:30
|g year:2014
|g number:22
|g day:10
|g month:06
|g pages:6602-11
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|3 Volltext
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