|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM238118029 |
| 003 |
DE-627 |
| 005 |
20231224113108.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.clim.2014.04.017
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0793.xml
|
| 035 |
|
|
|a (DE-627)NLM238118029
|
| 035 |
|
|
|a (NLM)24814239
|
| 035 |
|
|
|a (PII)S1521-6616(14)00125-9
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Demberg, Thorsten
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Phenotypes and distribution of mucosal memory B-cell populations in the SIV/SHIV rhesus macaque model
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 05.09.2014
|
| 500 |
|
|
|a Date Revised 21.10.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Published by Elsevier Inc.
|
| 520 |
|
|
|a As vaccine-elicited antibodies have now been associated with HIV protective efficacy, a thorough understanding of mucosal and systemic B-cell development and maturation is needed. We phenotyped mucosal memory B-cells, investigated isotype expression and homing patterns, and defined plasmablasts and plasma cells at three mucosal sites (duodenum, jejunum and rectum) in rhesus macaques, the commonly used animal model for pre-clinical vaccine studies. Unlike humans, macaque mucosal memory B-cells lacked CD27 expression; only two sub-populations were present: naïve (CD21(+)CD27(-)) and tissue-like (CD21(-)CD27(-)) memory. Similar to humans, IgA was the dominant isotype expressed. The homing markers CXCR4, CCR6, CCR9 and α4β7 were differentially expressed between naïve and tissue-like memory B-cells. Mucosal plasmablasts were identified as CD19(+)CD20(+/-)HLA-DR(+)Ki-67(+)IRF4(+)CD138(+/-) and mucosal plasma cells as CD19(+)CD20(-)HLA-DR(-)Ki-67(-)IRF4(+)CD138(+). Both populations were CD39(+/-)CD27(-). Plasma cell phenotype was confirmed by spontaneous IgA secretion by ELISpot of positively-selected cells and J-chain expression by real-time PCR. Duodenal, jejunal and rectal samples were similar in B-cell memory phenotype, isotype expression, homing receptors and plasmablast/plasma cell distribution among the three tissues. Thus rectal biopsies adequately monitor B-cell dynamics in the gut mucosa, and provide a critical view of mucosal B-cell events associated with development of vaccine-elicited protective immune responses and SIV/SHIV pathogenesis and disease control
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, N.I.H., Intramural
|
| 650 |
|
4 |
|a Homing markers
|
| 650 |
|
4 |
|a Mucosal memory B cell phenotypes and distribution
|
| 650 |
|
4 |
|a Plasmablasts/plasma cells
|
| 650 |
|
4 |
|a SIV/SHIV rhesus macaque model
|
| 650 |
|
7 |
|a Antigens, CD19
|2 NLM
|
| 650 |
|
7 |
|a Antigens, CD20
|2 NLM
|
| 650 |
|
7 |
|a Histocompatibility Antigens Class II
|2 NLM
|
| 650 |
|
7 |
|a Immunoglobulin A
|2 NLM
|
| 650 |
|
7 |
|a Interferon Regulatory Factors
|2 NLM
|
| 650 |
|
7 |
|a Ki-67 Antigen
|2 NLM
|
| 650 |
|
7 |
|a Receptors, Chemokine
|2 NLM
|
| 650 |
|
7 |
|a Syndecan-1
|2 NLM
|
| 650 |
|
7 |
|a interferon regulatory factor-4
|2 NLM
|
| 700 |
1 |
|
|a Mohanram, Venkatramanan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Venzon, David
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Robert-Guroff, Marjorie
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 153(2014), 2 vom: 01. Aug., Seite 264-76
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
|
| 773 |
1 |
8 |
|g volume:153
|g year:2014
|g number:2
|g day:01
|g month:08
|g pages:264-76
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.clim.2014.04.017
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_11
|
| 912 |
|
|
|a GBV_ILN_24
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 153
|j 2014
|e 2
|b 01
|c 08
|h 264-76
|