A simple filtration technique for obtaining purified human chromosomes in suspension

Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure human chromosomes were recovered using a simp...

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Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques. - 1991. - 56(2014), 5 vom: 09. Mai, Seite 257-61
1. Verfasser:	Yusuf, Mohammed (VerfasserIn)
Weitere Verfasser:	Parmar, Neha, Bhella, Gurdeep K, Robinson, Ian K
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't chromosome cytoplasmic debris filtration mitotic nuclei