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231224s2014 xx |||||o 00| ||eng c |
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7 |
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|a 10.1111/vcp.12150
|2 doi
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|a pubmed25n0793.xml
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|a (NLM)24798319
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Hillström, Anna
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein
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|c 2014
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 16.04.2015
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| 500 |
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|a Date Revised 10.11.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2014 The Authors Veterinary Clinical Pathology published by Wiley Periodicals, Inc. on behalf of American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Measurement of C-reactive protein (CRP) is used for diagnosing and monitoring systemic inflammatory disease in canine patients. An automated human immunoturbidimetric assay has been validated for measuring canine CRP, but cross-reactivity with canine CRP is unpredictable
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|a OBJECTIVE: The purpose of the study was to validate a new automated canine-specific immunoturbidimetric CRP method (Gentian cCRP)
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|a METHODS: Studies of imprecision, accuracy, prozone effect, interference, limit of quantification, and stability under different storage conditions were performed. The new method was compared with a human CRP assay previously validated for canine CRP determination. Samples from 40 healthy dogs were analyzed to establish a reference interval
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|a RESULTS: Total imprecision was < 2.4% for 4 tested serum pools analyzed twice daily over 10 days. The method was linear under dilution, and no prozone effect was detected at a concentration of 1200 mg/L. Recovery after spiking serum with purified canine CRP at 2 different concentrations was 123% and 116%, respectively. No interference from hemoglobin or triglycerides (10 g/L) was detected. CRP was stable for 14 days at 4°C and 22°C. In the method comparison study, there was good agreement between the validated human CRP assay and the new canine-specific assay. Healthy dogs had CRP concentrations that were less than the limit of quantification of the Gentian cCRP method (6.8 mg/L)
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|a CONCLUSIONS: The new canine-specific immunoturbidimetric CRP assay is a reliable and rapid method for measuring canine CRP, suitable for clinical use due to the option for an automated assay
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Validation Study
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|a Acute phase protein
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|a CRP
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|a method comparison
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| 650 |
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|a reference interval
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| 650 |
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4 |
|a stability
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| 650 |
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7 |
|a Acute-Phase Proteins
|2 NLM
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| 650 |
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|a C-Reactive Protein
|2 NLM
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| 650 |
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7 |
|a 9007-41-4
|2 NLM
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| 700 |
1 |
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|a Hagman, Ragnvi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tvedten, Harold
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kjelgaard-Hansen, Mads
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 43(2014), 2 vom: 15. Juni, Seite 235-43
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
1 |
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|g volume:43
|g year:2014
|g number:2
|g day:15
|g month:06
|g pages:235-43
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/vcp.12150
|3 Volltext
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