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231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la4047758
|2 doi
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|a pubmed24n0793.xml
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Saums, Michele K
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Mechanically and chemically tunable cell culture system for studying the myofibroblast phenotype
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 15.04.2015
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|a Date Revised 21.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Cell culture systems for studying the combined effects of matrix proteins and mechanical forces on the behavior of soft tissue cells have not been well developed. Here, we describe a new biomimetic cell culture system that allows for the study of mixtures of matrix proteins while controlling mechanical stiffness in a range that is physiological for soft tissues. This system consists of layer-by-layer (LbL)-assembled films of native matrix proteins atop mechanically tunable soft supports. We used hepatic stellate cells, which differentiate to myofibroblasts in liver fibrosis, for proof-of-concept studies. By culturing cells on collagen and lumican LbL-modified hydrogels, we demonstrate that this system is noncytotoxic and offers a valid control substrate, that the hydrogel determines the overall system mechanics, and that the addition of lumican to collagen influences the stellate cell phenotype. LbL-modified hydrogels offer the potential to study the influence of complex environmental factors on soft-tissue cells in culture
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Hydrogels
|2 NLM
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| 700 |
1 |
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|a Wang, Weifeng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Han, Biao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Madhavan, Lakshmi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Han, Lin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Daeyeon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wells, Rebecca G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 19 vom: 20. Mai, Seite 5481-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:30
|g year:2014
|g number:19
|g day:20
|g month:05
|g pages:5481-7
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|u http://dx.doi.org/10.1021/la4047758
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