|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM237627132 |
| 003 |
DE-627 |
| 005 |
20250216230453.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/eru164
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0792.xml
|
| 035 |
|
|
|a (DE-627)NLM237627132
|
| 035 |
|
|
|a (NLM)24759884
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Yuan, Hui
|e verfasserin
|4 aut
|
| 245 |
1 |
2 |
|a A novel gene, MdSSK1, as a component of the SCF complex rather than MdSBP1 can mediate the ubiquitination of S-RNase in apple
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 23.02.2015
|
| 500 |
|
|
|a Date Revised 21.10.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a As a core factor in S-RNase-based gametophytic self-incompatibility (GSI), the SCF (SKP1-Cullin1-F-box-Rbx1) complex (including pollen determinant SLF, S-locus-F-box) functions as an E3 ubiquitin ligase on non-self S-RNase. The SCF complex is formed by SKP1 bridging between SLF, CUL1, and Rbx1; however, it is not known whether an SCF complex lacking SKP1 can mediate the ubiquitination of S-RNase. Three SKP1-like genes from pollen were cloned based on the structural features of the SLF-interacting-SKP1-like (SSK) gene and the 'Golden Delicious' apple genome. These genes have a motif of five amino acids following the standard 'WAFE' at the C terminal and, in addition, contain eight sheets and two helices. All three genes were expressed exclusively in pollen. In the yeast two-hybrid and pull-down assays only one was found to interact with MdSFBB and MdCUL1, suggesting it is the SLF-interacting SKP1-like gene in apple which was named MdSSK1. In vitro experiments using MdSSK1, S2-MdSFBB1 (S2-Malus domestica S-locus-F-box brother) and MdCUL1 proteins incubated with S 2-RNase and ubiquitin revealed that the SCF complex ubiquitinylates S-RNase in vitro, while MdSBP1 (Malus domestica S-RNase binding protein 1) could not functionally replace MdSSK1 in the SCF complex in ubiquitinylating S-RNase. According to the above experiments, MdSBP1 is probably the only factor responsible for recognition with S-RNase, while not a component of the SCF complex, and an SCF complex containing MdSSK1 is required for mediating the ubiquitination of S-RNase
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Apple
|
| 650 |
|
4 |
|a S-RNase
|
| 650 |
|
4 |
|a SCF complex
|
| 650 |
|
4 |
|a SSK
|
| 650 |
|
4 |
|a self-incompatibility
|
| 650 |
|
4 |
|a ubiquitin.
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Ribonucleases
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.-
|2 NLM
|
| 700 |
1 |
|
|a Meng, Dong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Gu, Zhaoyu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Wei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Aide
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yang, Qing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhu, Yuandi
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Tianzhong
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 12 vom: 07. Juli, Seite 3121-31
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:65
|g year:2014
|g number:12
|g day:07
|g month:07
|g pages:3121-31
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/eru164
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 65
|j 2014
|e 12
|b 07
|c 07
|h 3121-31
|