Inactivation of an integrated antibiotic resistance gene in mammalian cells to re-enable antibiotic selection

Removing an antibiotic resistance gene allows the same antibiotic to be re-used in the next round of genetic manipulation. Here we applied the CRISPR/Cas system to disrupt the puromycin resistance gene in an engineered mouse embryonic stem cell line and then re-used puromycin selection in the result...

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Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques. - 1991. - 56(2014), 4 vom: 14., Seite 198-201
1. Verfasser:	Ni, Peiling (VerfasserIn)
Weitere Verfasser:	Zhang, Qian, Chen, Haixia, Chen, Lingyi
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't CRISPR/Cas antibiotic resistance gene genetic engineering Anti-Bacterial Agents Puromycin 4A6ZS6Q2CL