Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR
The viscosity of genomic DNA can interfere with digital PCR systems that partition samples into oil droplets or microfluidic wells. Restriction digestion may reduce the viscosity, but the process is labor-intensive, and the buffer can alter the conditions for PCR. DNA fragmentation using the QIAshre...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 56(2014), 4 vom: 14., Seite 194-6
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| 1. Verfasser: |
Yukl, Steven A
(VerfasserIn) |
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| Weitere Verfasser: |
Kaiser, Philipp,
Kim, Peggy,
Li, Peilin,
Wong, Joseph K |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
BsaJI
DNA
Droplet Digital PCR
QIAshredder
RsaI
digital PCR
mehr...
restriction enzyme
9007-49-2
DNA Restriction Enzymes
EC 3.1.21.- |
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