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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1021/la5004269
|2 doi
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|a pubmed24n0790.xml
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Palazon, Francisco
|e verfasserin
|4 aut
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|a Carbodiimide/NHS derivatization of COOH-terminated SAMs
|b activation or byproduct formation?
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 15.04.2015
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|a Date Revised 29.04.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a COOH-terminated self-assembled monolayers (SAMs) are widely used in biosensor technology to bind different amine-containing biomolecules. A covalent amide bond, however, can be achieved only if the carboxylic acids are activated. This activation process usually consists of forming an N-hydroxysuccinimidyl ester (NHS-ester) by consecutively reacting carboxylic acids with a carbodiimide and NHS. Though many papers report using this method,1-8 the experimental conditions vary greatly between them and chemical characterization at this stage is often omitted. Evidence of an efficient activation is therefore rarely shown. Furthermore, recent publications9-11 have highlighted the complexity of this process, with the possible formation of different byproducts. In this paper, we have conducted a study on NHS activation under different conditions with chemical characterization by polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS) and time-of-flight secondary ion mass spectroscopy (ToF-SIMS). Our results indicate that the nature of the solvent and carbodiimide and the reactant concentrations play crucial roles in activation kinetics and efficiency
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carbodiimides
|2 NLM
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|a Carboxylic Acids
|2 NLM
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|a Solvents
|2 NLM
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|a Benavides, Cindy Montenegro
|e verfasserin
|4 aut
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|a Léonard, Didier
|e verfasserin
|4 aut
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|a Souteyrand, Éliane
|e verfasserin
|4 aut
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|a Chevolot, Yann
|e verfasserin
|4 aut
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|a Cloarec, Jean-Pierre
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 16 vom: 29. Apr., Seite 4545-50
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:30
|g year:2014
|g number:16
|g day:29
|g month:04
|g pages:4545-50
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|u http://dx.doi.org/10.1021/la5004269
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