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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.12250
|2 doi
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|a (NLM)24716830
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Högfors-Rönnholm, E
|e verfasserin
|4 aut
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| 245 |
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|a Adhesion of smooth and rough phenotypes of Flavobacterium psychrophilum to polystyrene surfaces
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|c 2015
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 23.06.2015
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2014 John Wiley & Sons Ltd.
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|a Phenotypic smooth cells of the fish pathogenic bacterium Flavobacterium psychrophilum have previously been reported to be more adhesive to polystyrene surfaces than corresponding rough cells. In this study, the adhesion ability of smooth and rough cells of F. psychrophilum to polystyrene surfaces was investigated in detail with a crystal violet staining method. By treating both polystyrene surfaces with fish mucus and carbohydrates and the bacterial cells with carbohydrates, the involvement of lectins in the adhesion process was investigated. Smooth cells showed significantly higher adhesion ability to untreated polystyrene surfaces compared with corresponding rough cells and increasing water hardness had an inhibitory effect on the adhesion. Treatment of polystyrene surfaces with D-glucose, D-galactose and fish mucus increased the adhesion ability of smooth cells to polystyrene. Furthermore, treatment of the smooth cells with D-glucose, D-galactose and sialic acid decreased the adhesion ability of the cells, indicating that the adhesion is likely mediated by complementary lectins on the surface of the cells. Sodium (meta)periodate treatment of smooth cells also decreased the adhesion ability to polystyrene, suggesting that the lectins, such as the dominating sialic acid-binding lectin, are probably localized in the extracellular polysaccharides surrounding the cells
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Flavobacterium psychrophilum
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|a adhesion
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|a lectins
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|a phase variation
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|a phenotype
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|a Lectins
|2 NLM
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|a Polystyrenes
|2 NLM
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|a Periodic Acid
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|a 10450-60-9
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|a metaperiodate
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| 700 |
1 |
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|a Norrgård, J
|e verfasserin
|4 aut
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1 |
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|a Wiklund, T
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 38(2015), 5 vom: 10. Mai, Seite 429-37
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
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|g volume:38
|g year:2015
|g number:5
|g day:10
|g month:05
|g pages:429-37
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|u http://dx.doi.org/10.1111/jfd.12250
|3 Volltext
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