Quantitative phenotyping of leaf margins in three dimensions, demonstrated on KNOTTED and TCP trangenics in Arabidopsis

The geometry of leaf margins is an important shape characteristic that distinguishes among different leaf phenotypes. Current definitions of leaf shape are qualitative and do not allow quantification of differences in shape between phenotypes. This is especially true for leaves with some non-trivial...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 65(2014), 8 vom: 04. Mai, Seite 2071-7
1. Verfasser: Armon, Shahaf (VerfasserIn)
Weitere Verfasser: Yanai, Osnat, Ori, Naomi, Sharon, Eran
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis curvature differential geometry growth leaf shape lobes waviness. Homeodomain Proteins mehr... Kn1 protein, plant Plant Proteins Transcription Factors Dexamethasone 7S5I7G3JQL
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520 |a The geometry of leaf margins is an important shape characteristic that distinguishes among different leaf phenotypes. Current definitions of leaf shape are qualitative and do not allow quantification of differences in shape between phenotypes. This is especially true for leaves with some non-trivial three-dimensional (3D) configurations. Here we present a novel geometrical method novel geometrical methods to define, measure, and quantify waviness and lobiness of leaves. The method is based on obtaining the curve of the leaf rim from a 3D surface measurement and decomposing its local curvature vector into the normal and geodesic components. We suggest that leaf waviness is associated with oscillating normal curvature along the margins, while lobiness is associated with oscillating geodesic curvature. We provide a way to integrate these local measures into global waviness and lobiness quantities. Using these novel definitions, we analysed the changes in leaf shape of two Arabidopsis genotypes, either as a function of gene mis-expression induction level or as a function of time. These definitions and experimental methods open the way for a more quantitative study of the shape of leaves and other growing slender organs 
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700 1 |a Yanai, Osnat  |e verfasserin  |4 aut 
700 1 |a Ori, Naomi  |e verfasserin  |4 aut 
700 1 |a Sharon, Eran  |e verfasserin  |4 aut 
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