Identification of predominant genes involved in regulation and execution of senescence-associated nitrogen remobilization in flag leaves of field grown barley

© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 65(2014), 14 vom: 12. Juli, Seite 3963-73
1. Verfasser: Hollmann, Julien (VerfasserIn)
Weitere Verfasser: Gregersen, Per L, Krupinska, Karin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Barley Hordeum vulgare L. HvNAC026. field experiment flag leaf leaf senescence nitrogen remobilization nitrogen supply mehr... Nitrogen N762921K75
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520 |a The transcriptomes of senescing flag leaves collected from barley field plots with standard or high nitrogen supply were compared to identify genes specifically associated with nitrogen remobilization during leaf senescence under agronomically relevant conditions. In flag leaves collected in field plots with high nitrogen supply, the decline in chlorophyll content was delayed. By comparing changes in gene expression for the two nitrogen levels, it was possible to discriminate genes related to nitrogen remobilization during senescence and genes involved in other processes associated with the late development of leaves under field conditions. Predominant genes that were more strongly upregulated during senescence of flag leaves from plants with standard nitrogen supply included genes encoding the transcription factor HvNAC026, serine type protease SCPL51, and the autophagy factors APG7 and ATG18F. Elevated expression of these genes in senescing leaves from plants with standard nitrogen supply indicates important roles of the corresponding proteins in nitrogen remobilization. In comparison, the genes upregulated in both flag leaf samples might have roles in general senescence processes associated with late leaf development. Among these genes were the transcription factor genes HvNAC001, HvNAC005, HvNAC013, HvWRKY12 and MYB, genes encoding the papain-like cysteine peptidases HvPAP14 and HvPAP20, as well as a subtilase gene 
650 4 |a Journal Article 
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650 4 |a Hordeum vulgare L. 
650 4 |a HvNAC026. 
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650 4 |a flag leaf 
650 4 |a leaf senescence 
650 4 |a nitrogen remobilization 
650 4 |a nitrogen supply 
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700 1 |a Gregersen, Per L  |e verfasserin  |4 aut 
700 1 |a Krupinska, Karin  |e verfasserin  |4 aut 
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