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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/eru142
|2 doi
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|a pubmed24n0790.xml
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|a (DE-627)NLM236985639
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|a (NLM)24692654
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Lindner, Ann-Cathrin
|e verfasserin
|4 aut
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|a Isopentenyltransferase-1 (IPT1) knockout in Physcomitrella together with phylogenetic analyses of IPTs provide insights into evolution of plant cytokinin biosynthesis
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 09.01.2015
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|a Date Revised 21.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a The moss Physcomitrella patens is part of an early divergent clade of land plants utilizing the plant hormone cytokinin for growth control. The rate-limiting step of cytokinin biosynthesis is mediated by isopentenyltransferases (IPTs), found in land plants either as adenylate-IPTs or as tRNA-IPTs. Although a dominant part of cytokinins in flowering plants are synthesized by adenylate-IPTs, the Physcomitrella genome only encodes homologues of tRNA-IPTs. This study therefore looked into the question of whether cytokinins in moss derive from tRNA exclusively. Targeted gene knockout of ipt1 (d|ipt1) along with localization studies revealed that the chloroplast-bound IPT1 was almost exclusively responsible for the A37 prenylation of tRNA in Physcomitrella. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based cytokinin profiling demonstrated that the total amount of all free cytokinins in tissue was almost unaffected. However, the knockout plants showed increased levels of the N (6) -isopentenyladenine (iP)- and trans-zeatin (tZ)-type cytokinins, considered to provide active forms, while cis-zeatin (cZ)-type cytokinins were reduced. The data provide evidence for an additional and unexpected tRNA-independent cytokinin biosynthetic pathway in moss. Comprehensive phylogenetic analysis indicates a diversification of tRNA-IPT-like genes in bryophytes probably related to additional functions
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bryophyte
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|a cytokinin
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|a isopentenyladenosine
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|a isopentenyltransferases
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|a moss
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|a tRNA.
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|a Cytokinins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a RNA, Transfer
|2 NLM
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|a 9014-25-9
|2 NLM
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|a Alkyl and Aryl Transferases
|2 NLM
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|a EC 2.5.-
|2 NLM
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|a adenylate isopentenyltransferase
|2 NLM
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|a EC 2.5.1.27
|2 NLM
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|a Lang, Daniel
|e verfasserin
|4 aut
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|a Seifert, Maike
|e verfasserin
|4 aut
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| 700 |
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|a Podlešáková, Kateřina
|e verfasserin
|4 aut
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| 700 |
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|a Novák, Ondřej
|e verfasserin
|4 aut
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|a Strnad, Miroslav
|e verfasserin
|4 aut
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|a Reski, Ralf
|e verfasserin
|4 aut
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|a von Schwartzenberg, Klaus
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 9 vom: 01. Juni, Seite 2533-43
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:65
|g year:2014
|g number:9
|g day:01
|g month:06
|g pages:2533-43
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|u http://dx.doi.org/10.1093/jxb/eru142
|3 Volltext
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|d 65
|j 2014
|e 9
|b 01
|c 06
|h 2533-43
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