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231224s2014 xx |||||o 00| ||eng c |
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7 |
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|a 10.1021/la404673q
|2 doi
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|a eng
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| 100 |
1 |
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|a Zeng, Guanghong
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Single-cell force spectroscopy of bacteria enabled by naturally derived proteins
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 10.04.2015
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|a Date Revised 15.04.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Bringing the study of bacterial adhesion down to a single-cell level is critical for understanding the molecular mechanisms involved in initial bacterial attachment. We have developed a simple and versatile method for making single-cell bacterial probes to study the adhesion of single bacterial cells by atomic force microscopy (AFM). A single-cell probe was made by picking up a bacterial cell from a glass surface using a tipless AFM cantilever coated with a commercial cell adhesive Cell-Tak. The method was applied to four different bacterial strains, and single-cell adhesion was measured on three surfaces (fresh glass, hydrophilic glass, and mica). Attachment to the cantilever was stable during the AFM force measurements that were conducted for 2 h, and viability was confirmed by Live/Dead fluorescence staining at the end of each experiment. The adhesion force and final rupture length were dependent on bacterial strains, surfaces properties, and contact time. The single-cell probe offers control of cell immobilization and thus holds advantages over the commonly used multicell probes with which random immobilization is obtained by submerging the cantilever in a bacterial suspension. The reported method provides a general platform for investigating single-cell interactions of bacteria with different surfaces and other cells by AFM force spectroscopy, thus improving our understanding of the mechanisms of bacterial attachment
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Bacterial Proteins
|2 NLM
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| 700 |
1 |
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|a Müller, Torsten
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Meyer, Rikke L
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 14 vom: 15. Apr., Seite 4019-25
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|g volume:30
|g year:2014
|g number:14
|g day:15
|g month:04
|g pages:4019-25
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|u http://dx.doi.org/10.1021/la404673q
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