t-PA acts as a cytokine to regulate lymphocyte-endothelium adhesion in experimental autoimmune encephalomyelitis

t-PA acts as a cytokine to regulate lymphocyte-endothelium adhesion in experimental autoimmune encephalomyelitis

Copyright © 2014 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 152(2014), 1-2 vom: 01. Mai, Seite 90-100
1. Verfasser: Wang, Jinghua (VerfasserIn)
Weitere Verfasser: Zhang, Xin, Mu, Lili, Zhang, Mingqing, Gao, Zhongming, Zhang, Jia, Yao, Xiuhua, Liu, Chuanliang, Wang, Guangyou, Wang, Dandan, Kong, Qingfei, Liu, Yumei, Li, Na, Sun, Bo, Li, Hulun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Endothelial cells Experimental autoimmune encephalomyelitis ICAM-1 LRP1 Tissue-type plasminogen activator Antibodies, Monoclonal Cytokines Low Density Lipoprotein Receptor-Related Protein-1 mehr... Lrp1 protein, mouse Receptors, LDL Tumor Suppressor Proteins Intercellular Adhesion Molecule-1 126547-89-5 Cyclic AMP E0399OZS9N Cyclic AMP-Dependent Protein Kinases EC 2.7.11.11 Tissue Plasminogen Activator EC 3.4.21.68
Beschreibung
Zusammenfassung:Copyright © 2014 Elsevier Inc. All rights reserved.
In this study, the capacity for t-PA to affect T cell-brain microvascular endothelial cell adhesion by acting as a cytokine was investigated. Following the treatment of a brain-derived endothelial cell line, bEnd.3, with various concentrations of t-PA, adhesion and transwell migration assays were performed. In the presence of t-PA, enhanced adhesion of T cells to bEnd.3 cells was observed. Using western blot analysis, an increase in ICAM-1 expression was detected for both t-PA-treated bEnd.3 cells and bEnd.3 cells treated with a non-enzymatic form of t-PA. In contrast, when LRP1 was blocked using a specific antibody, upregulation of ICAM-1 was inhibited and cAMP-PKA signaling was affected. Furthermore, using an EAE mouse model, administration of t-PA was associated with an increase in ICAM-1 expression by brain endothelial cells. Taken together, these findings suggest that t-PA can induce ICAM-1 expression in brain microvascular endothelial cells, and this may promote the development of EAE
Beschreibung:Date Completed 02.07.2014
Date Revised 09.12.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2014.03.004