|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM236455656 |
| 003 |
DE-627 |
| 005 |
20231224105617.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/la5001396
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0788.xml
|
| 035 |
|
|
|a (DE-627)NLM236455656
|
| 035 |
|
|
|a (NLM)24635537
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Adil, Maroof M
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Transfection mechanisms of polyplexes, lipoplexes, and stealth liposomes in α₅β₁ integrin bearing DLD-1 colorectal cancer cells
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 13.04.2015
|
| 500 |
|
|
|a Date Revised 25.11.2016
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Receptor targeted, PEGylated transfection agents can improve stability and delivery specificity of current cationic lipid and polymer based nonviral gene delivery vehicles, but their mode of transfection is poorly understood. We therefore investigated the transfection mechanisms of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP)/1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) lipoplexes, branched polyethylenimine (bPEI) polyplexes, and bPEI encapsulated in either PEGylated (stealth) nontargeted liposomes or PR_b peptide (targeted to α5β1 integrin) functionalized stealth liposomes in DLD-1 colorectal cancer cells in vitro with gene expression assays, flow cytometry and confocal microscopy. DOTAP/DOPE and PR_b functionalized stealth liposomes mediated higher gene expression compared to nontargeted stealth liposomes and bPEI. However DOTAP/DOPE was internalized slowly leading to lower levels of DNA uptake. In contrast, despite high internalization of bPEI polyplexes, gene expression levels were low as DNA was unable to escape from the endosomes. Nontargeted stealth liposomes also mediated low gene expression due to low amounts of DNA internalized and slow internalization kinetics. PR_b functionalized stealth liposomes struck an optimal balance among these transfection agents with efficient transfection arising from fast integrin mediated internalization kinetics, high amounts of DNA uptake, and endosomal escape. We found α5β1 integrin to be a valuable target for gene delivery and that the caveolar endocytic pathway may offer an advantage to receptor targeted PEGylated transfection agents in DLD-1 cells
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Fatty Acids, Monounsaturated
|2 NLM
|
| 650 |
|
7 |
|a Integrin alpha5beta1
|2 NLM
|
| 650 |
|
7 |
|a Liposomes
|2 NLM
|
| 650 |
|
7 |
|a Phosphatidylethanolamines
|2 NLM
|
| 650 |
|
7 |
|a Quaternary Ammonium Compounds
|2 NLM
|
| 650 |
|
7 |
|a 1,2-dielaidoylphosphatidylethanolamine
|2 NLM
|
| 650 |
|
7 |
|a 76391-83-8
|2 NLM
|
| 650 |
|
7 |
|a Polyethyleneimine
|2 NLM
|
| 650 |
|
7 |
|a 9002-98-6
|2 NLM
|
| 650 |
|
7 |
|a Luciferases
|2 NLM
|
| 650 |
|
7 |
|a EC 1.13.12.-
|2 NLM
|
| 650 |
|
7 |
|a 1,2-dioleoyloxy-3-(trimethylammonium)propane
|2 NLM
|
| 650 |
|
7 |
|a MR86K0XRQP
|2 NLM
|
| 700 |
1 |
|
|a Erdman, Zachary S
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kokkoli, Efrosini
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 13 vom: 08. Apr., Seite 3802-10
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:30
|g year:2014
|g number:13
|g day:08
|g month:04
|g pages:3802-10
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/la5001396
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 30
|j 2014
|e 13
|b 08
|c 04
|h 3802-10
|