Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood

Bibliographische Detailangaben
Veröffentlicht in:	Clinical immunology (Orlando, Fla.). - 1999. - 152(2014), 1-2 vom: 15. Mai, Seite 20-4
1. Verfasser:	Kuerten, Stefanie (VerfasserIn)
Weitere Verfasser:	Pommerschein, Giovanna, Barth, Stefanie K, Hohmann, Christopher, Milles, Bianca, Sammer, Fabian W, Duffy, Cathrina E, Wunsch, Marie, Rovituso, Damiano M, Schroeter, Michael, Addicks, Klaus, Kaiser, Claudia C, Lehmann, Paul V
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	Clinical immunology (Orlando, Fla.)
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Autoimmunity; B cells; Blood; ELISPOT; Multiple sclerosis

Beschreibung
Zusammenfassung:	Copyright © 2014. Published by Elsevier Inc. B cells are increasingly coming into play in the pathogenesis of multiple sclerosis (MS). Here, we screened peripheral blood mononuclear cells (PBMC) from patients with clinically isolated syndrome (CIS), MS, other non-inflammatory neurological, inflammatory neurological or autoimmune diseases, and healthy donors for their B cell reactivity to CNS antigen using the enzyme-linked immunospot technique (ELISPOT) after 96 h of polyclonal stimulation. Our data show that nine of 15 patients with CIS (60.0%) and 53 of 67 patients with definite MS (79.1%) displayed CNS-reactive B cells, compared to none of the control donors. The presence of CNS-reactive B cells in the blood of the majority of patients with MS or at risk to develop MS along with their absence in control subjects suggests that they might be indicative of a B cell-dependent subpopulation of the disease
Beschreibung:	Date Completed 02.07.2014 Date Revised 28.04.2014 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-7035
DOI:	10.1016/j.clim.2014.02.014