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20231224104916.0 |
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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/eru055
|2 doi
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|a pubmed24n0787.xml
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|a (DE-627)NLM236134744
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|a (NLM)24600022
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Iglesias-Fernández, Raquel
|e verfasserin
|4 aut
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| 245 |
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|a The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 24.12.2014
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|a Date Revised 21.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Protein hydrolysis plays an important role during seed germination and post-germination seedling establishment. In Arabidopsis thaliana, cathepsin B-like proteases are encoded by a gene family of three members, but only the AtCathB3 gene is highly induced upon seed germination and at the early post-germination stage. Seeds of a homozygous T-DNA insertion mutant in the AtCathB3 gene have, besides a reduced cathepsin B activity, a slower germination than the wild type. To explore the transcriptional regulation of this gene, we used a combined phylogenetic shadowing approach together with a yeast one-hybrid screening of an arrayed library of approximately 1200 transcription factor open reading frames from Arabidopsis thaliana. We identified a conserved CathB3-element in the promoters of orthologous CathB3 genes within the Brassicaceae species analysed, and, as its DNA-interacting protein, the G-Box Binding Factor1 (GBF1). Transient overexpression of GBF1 together with a PAtCathB3::uidA (β-glucuronidase) construct in tobacco plants revealed a negative effect of GBF1 on expression driven by the AtCathB3 promoter. In stable P35S::GBF1 lines, not only was the expression of the AtCathB3 gene drastically reduced, but a significant slower germination was also observed. In the homozygous knockout mutant for the GBF1 gene, the opposite effect was found. These data indicate that GBF1 is a transcriptional repressor of the AtCathB3 gene and affects the germination kinetics of Arabidopsis thaliana seeds. As AtCathB3 is also expressed during post-germination in the cotyledons, a role for the AtCathB3-like protease in reserve mobilization is also inferred
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis thaliana
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|a AtCathB3
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|a GBF1
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|a bZIP
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|a cathepsin B-like enzymes
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|a phylogenetic shadowing
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|a reserve mobilization
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|a seed germination
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|a transcriptional regulation.
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a GBF1 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 650 |
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|a Glucuronidase
|2 NLM
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| 650 |
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|a EC 3.2.1.31
|2 NLM
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| 650 |
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|a Cathepsin B
|2 NLM
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| 650 |
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|a EC 3.4.22.1
|2 NLM
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| 700 |
1 |
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|a Wozny, Dorothee
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Iriondo-de Hond, Maite
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Oñate-Sánchez, Luis
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Carbonero, Pilar
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Barrero-Sicilia, Cristina
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 8 vom: 18. Mai, Seite 2009-21
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:65
|g year:2014
|g number:8
|g day:18
|g month:05
|g pages:2009-21
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/eru055
|3 Volltext
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|d 65
|j 2014
|e 8
|b 18
|c 05
|h 2009-21
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