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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2013.11.009
|2 doi
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|a pubmed24n0787.xml
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|a (DE-627)NLM236081136
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|a (NLM)24594388
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|a (PII)S0176-1617(13)00454-9
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Rubio, Sebastián
|e verfasserin
|4 aut
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|a The dormancy-breaking stimuli "chilling, hypoxia and cyanamide exposure" up-regulate the expression of α-amylase genes in grapevine buds
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.11.2014
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|a Date Revised 08.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier GmbH. All rights reserved.
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|a It has been suggested that respiratory stress is involved in the mechanism underlying the dormancy-breaking effect of hydrogen cyanamide (H2CN2) and sodium azide in grapevine buds; indeed, reductions in oxygen levels (hypoxia) and inhibitors of respiration promote bud-break in grapevines. In this study, we showed that, hypoxia increased starch hydrolysis soluble sugar consumption and up-regulated the expression of α-amylase genes (Vvα-AMYs) in grapevine buds, suggesting that these biochemical changes induced by hypoxia, may play a relevant role in the release of buds from endodormancy (ED). Three of the four Vvα-AMY genes that are expressed in grapevine buds were up-regulated by hypoxia and a correlation between changes in sugar content and level of Vvα-AMY gene expression during the hypoxia treatment was found, suggesting that soluble sugars mediate the effect of hypoxia on Vvα-AMY gene expression. Exogenous applications of soluble sugars and sugar analogs confirmed this finding and revealed that osmotic stress induces the expression of Vvα-AMY1 and Vvα-AMY3 and that soluble sugars induces Vvα-AMY2 and Vvα-AMY4 gene expression. Interestingly, the plant hormone gibberellic acid (GA3) induced the expression of Vvα-AMY3 and Vvα-AMY4 genes, while dormancy breaking stimuli, chilling and cyanamide exposure, mainly induced the expression of Vvα-AMY1 and Vvα-AMY2 genes, suggesting that these two α-amylase genes might be involved in the release of grapevine buds from the ED
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Grapevine buds
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|a Hydrogen cyanamide
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|a Hypoxia
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|a Sugar signaling
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|a α-Amylase
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|a Carbohydrates
|2 NLM
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|a Gibberellins
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|a Plant Growth Regulators
|2 NLM
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|a Plant Proteins
|2 NLM
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|a RNA, Plant
|2 NLM
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|a Cyanamide
|2 NLM
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|a 420-04-2
|2 NLM
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|a Starch
|2 NLM
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|a 9005-25-8
|2 NLM
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|a gibberellic acid
|2 NLM
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|a BU0A7MWB6L
|2 NLM
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|a alpha-Amylases
|2 NLM
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|a EC 3.2.1.1
|2 NLM
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|a Oxygen
|2 NLM
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|a S88TT14065
|2 NLM
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|a Donoso, Amanda
|e verfasserin
|4 aut
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|a Pérez, Francisco J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 171(2014), 6 vom: 15. März, Seite 373-81
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:171
|g year:2014
|g number:6
|g day:15
|g month:03
|g pages:373-81
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| 856 |
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|u http://dx.doi.org/10.1016/j.jplph.2013.11.009
|3 Volltext
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|a AR
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|d 171
|j 2014
|e 6
|b 15
|c 03
|h 373-81
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