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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ert480
|2 doi
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|a pubmed25n0786.xml
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|a (DE-627)NLM235895725
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|a (NLM)24574485
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Butt, Haroon
|e verfasserin
|4 aut
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| 245 |
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|a Expression analysis of Arabidopsis XH/XS-domain proteins indicates overlapping and distinct functions for members of this gene family
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 04.11.2014
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|a Date Revised 11.12.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a RNA-directed DNA methylation (RdDM) is essential for de novo DNA methylation in higher plants, and recent reports established novel elements of this silencing pathway in the model organism Arabidopsis thaliana. Involved in de novo DNA methylation 2 (IDN2) and the closely related factor of DNA methylation (FDM) are members of a plant-specific family of dsRNA-binding proteins characterized by conserved XH/XS domains and implicated in the regulation of RdDM at chromatin targets. Genetic analyses have suggested redundant as well as non-overlapping activities for different members of the gene family. However, detailed insights into the function of XH/XS-domain proteins are still elusive. By the generation and analysis of higher-order mutant combinations affected in IDN2 and further members of the gene family, we have provided additional evidence for their redundant activity. Distinct roles for members of the XH/XS-domain gene family were indicated by differences in their expression and subcellular localization. Fluorescent protein-tagged FDM genes were expressed either in nuclei or in the cytoplasm, suggestive of activities of XH/XS-domain proteins in association with chromatin as well as outside the nuclear compartment. In addition, we observed altered location of a functional FDM1-VENUS reporter from the nucleus into the cytoplasm under conditions when availability of further FDM proteins was limited. This is suggestive of a mechanism by which redistribution of XH/XS-domain proteins could compensate for the loss of closely related proteins
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis
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|a RdDM.
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|a gene family
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|a protein localization
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|a Arabidopsis Proteins
|2 NLM
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|a FDM1 protein, Arabidopsis
|2 NLM
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| 650 |
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|a IDN2 protein, Arabidopsis
|2 NLM
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| 650 |
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|a RNA, Small Interfering
|2 NLM
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| 650 |
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|a RNA-Binding Proteins
|2 NLM
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| 700 |
1 |
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|a Graner, Sonja
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luschnig, Christian
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 4 vom: 26. März, Seite 1217-27
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:65
|g year:2014
|g number:4
|g day:26
|g month:03
|g pages:1217-27
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|u http://dx.doi.org/10.1093/jxb/ert480
|3 Volltext
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|d 65
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|h 1217-27
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