Physicochemical aspects of lipase B from Candida antarctica in bicontinuous microemulsions

Biotechnology involves applying enzymes in organic synthesis to convert non-natural substrates into enantiomerically pure products under mild reaction conditions. Non-natural substrates are often lipophilic molecules that can hardly be accessed and converted by enzymes in their natural aqueous envir...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 30(2014), 11 vom: 25. März, Seite 2993-3000
1. Verfasser: Subinya, Mireia (VerfasserIn)
Weitere Verfasser: Steudle, Anne K, Nestl, Bettina, Nebel, Bernd, Hauer, Bernhard, Stubenrauch, Cosima, Engelskirchen, Sandra
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Emulsions Ethers Fungal Proteins Octanes Water 059QF0KO0R pentaethylene glycol monododecyl ether 3055-95-6 mehr... Polyethylene Glycols 3WJQ0SDW1A Sodium Chloride 451W47IQ8X Lipase EC 3.1.1.3 lipase B, Candida antarctica octane X1RV0B2FJV
Beschreibung
Zusammenfassung:Biotechnology involves applying enzymes in organic synthesis to convert non-natural substrates into enantiomerically pure products under mild reaction conditions. Non-natural substrates are often lipophilic molecules that can hardly be accessed and converted by enzymes in their natural aqueous environment. Bicontinuous microemulsions provide a spongelike nanostructure with a large interfacial area between aqueous and oil domains, which makes them valuable alternative reaction media. In the present study, we introduced lipase B from Candida antarctica into a bicontinuous microemulsion of composition H2O/NaCl-n-octane-pentaethylene glycol monodecylether (C10E5). Phase behavior, partitioning studies, and pulsed-field-gradient NMR measurements revealed that the lipase is mostly adsorbed at the microemulsions interface. Phase diagrams showed a maximum in efficiency with increasing amount of lipase added to the water phase of the microemulsion. It was observed that the ratio between the mass of lipase that is introduced into the system and the mass of lipase that is located at the interface stays constant. Self-diffusion coefficients of all components showed that the presence of the lipase is not influencing the bicontinuity of the microemulsion
Beschreibung:Date Completed 12.11.2014
Date Revised 02.12.2018
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la4042088