High-throughput sequencing of natively paired antibody chains provides evidence for original antigenic sin shaping the antibody response to influenza vaccination

High-throughput sequencing of natively paired antibody chains provides evidence for original antigenic sin shaping the antibody response to influenza vaccination

Published by Elsevier Inc.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 151(2014), 1 vom: 01. März, Seite 55-65
1. Verfasser: Tan, Yann-Chong (VerfasserIn)
Weitere Verfasser: Blum, Lisa K, Kongpachith, Sarah, Ju, Chia-Hsin, Cai, Xiaoyong, Lindstrom, Tamsin M, Sokolove, Jeremy, Robinson, William H
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Antibody; B cell Influenza; Antibodies, Neutralizing Antibodies, Viral Antigens, Viral Hemagglutinins, Viral mehr... Immunoglobulin G Immunoglobulin Heavy Chains Immunoglobulin Light Chains Influenza Vaccines Vaccines, Subunit
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245 1 0 |a High-throughput sequencing of natively paired antibody chains provides evidence for original antigenic sin shaping the antibody response to influenza vaccination 
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500 |a Date Revised 21.10.2021 
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500 |a Citation Status MEDLINE 
520 |a Published by Elsevier Inc. 
520 |a We developed a DNA barcoding method to enable high-throughput sequencing of the cognate heavy- and light-chain pairs of the antibodies expressed by individual B cells. We used this approach to elucidate the plasmablast antibody response to influenza vaccination. We show that >75% of the rationally selected plasmablast antibodies bind and neutralize influenza, and that antibodies from clonal families, defined by sharing both heavy-chain VJ and light-chain VJ sequence usage, do so most effectively. Vaccine-induced heavy-chain VJ regions contained on average >20 nucleotide mutations as compared to their predicted germline gene sequences, and some vaccine-induced antibodies exhibited higher binding affinities for hemagglutinins derived from prior years' seasonal influenza as compared to their affinities for the immunization strains. Our results show that influenza vaccination induces the recall of memory B cells that express antibodies that previously underwent affinity maturation against prior years' seasonal influenza, suggesting that 'original antigenic sin' shapes the antibody response to influenza vaccination 
650 4 |a Journal Article 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Antibody; 
650 4 |a B cell 
650 4 |a Influenza; 
650 7 |a Antibodies, Neutralizing  |2 NLM 
650 7 |a Antibodies, Viral  |2 NLM 
650 7 |a Antigens, Viral  |2 NLM 
650 7 |a Hemagglutinins, Viral  |2 NLM 
650 7 |a Immunoglobulin G  |2 NLM 
650 7 |a Immunoglobulin Heavy Chains  |2 NLM 
650 7 |a Immunoglobulin Light Chains  |2 NLM 
650 7 |a Influenza Vaccines  |2 NLM 
650 7 |a Vaccines, Subunit  |2 NLM 
700 1 |a Blum, Lisa K  |e verfasserin  |4 aut 
700 1 |a Kongpachith, Sarah  |e verfasserin  |4 aut 
700 1 |a Ju, Chia-Hsin  |e verfasserin  |4 aut 
700 1 |a Cai, Xiaoyong  |e verfasserin  |4 aut 
700 1 |a Lindstrom, Tamsin M  |e verfasserin  |4 aut 
700 1 |a Sokolove, Jeremy  |e verfasserin  |4 aut 
700 1 |a Robinson, William H  |e verfasserin  |4 aut 
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856 4 0 |u http://dx.doi.org/10.1016/j.clim.2013.12.008  |3 Volltext 
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