Field resistance to Fusarium oxysporum and Verticillium dahliae in transgenic cotton expressing the plant defensin NaD1

The plant defensin NaD1, from Nicotiana alata, has potent antifungal activity against a range of filamentous fungi including the two important cotton pathogens, Fusarium oxysporum f. sp. vasinfectum (Fov) and Verticillium dahliae. Transgenic cotton plants expressing NaD1 were produced and plants fro...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 65(2014), 6 vom: 07. Apr., Seite 1541-50
1. Verfasser: Gaspar, Yolanda M (VerfasserIn)
Weitere Verfasser: McKenna, James A, McGinness, Bruce S, Hinch, Jillian, Poon, Simon, Connelly, Angela A, Anderson, Marilyn A, Heath, Robyn L
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Antifungal Fusarium oxysporum Verticillium dahliae. defensin field trials transgenic cotton Defensins Plant Proteins
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520 |a The plant defensin NaD1, from Nicotiana alata, has potent antifungal activity against a range of filamentous fungi including the two important cotton pathogens, Fusarium oxysporum f. sp. vasinfectum (Fov) and Verticillium dahliae. Transgenic cotton plants expressing NaD1 were produced and plants from three events were selected for further characterization. Homozygous plants were assessed in greenhouse bioassays for resistance to Fov. One line (D1) was selected for field trial testing over three growing seasons in soils naturally infested with Fov and over two seasons in soils naturally infested with V. dahliae. In the field trials with Fov-infested soil, line D1 had 2-3-times the survival rate, a higher tolerance to Fov (higher disease rank), and a 2-4-fold increase in lint yield compared to the non-transgenic Coker control. When transgenic line D1 was planted in V. dahliae-infested soil, plants had a higher tolerance to Verticillium wilt and up to a 2-fold increase in lint yield compared to the non-transgenic Coker control. Line D1 did not exhibit any detrimental agronomic features compared to the parent Coker control when plants were grown in non-diseased soil. This study demonstrated that the expression of NaD1 in transgenic cotton plants can provide substantial resistance to two economically important fungal pathogens 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Antifungal 
650 4 |a Fusarium oxysporum 
650 4 |a Verticillium dahliae. 
650 4 |a defensin 
650 4 |a field trials 
650 4 |a transgenic cotton 
650 7 |a Defensins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a McKenna, James A  |e verfasserin  |4 aut 
700 1 |a McGinness, Bruce S  |e verfasserin  |4 aut 
700 1 |a Hinch, Jillian  |e verfasserin  |4 aut 
700 1 |a Poon, Simon  |e verfasserin  |4 aut 
700 1 |a Connelly, Angela A  |e verfasserin  |4 aut 
700 1 |a Anderson, Marilyn A  |e verfasserin  |4 aut 
700 1 |a Heath, Robyn L  |e verfasserin  |4 aut 
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773 1 8 |g volume:65  |g year:2014  |g number:6  |g day:07  |g month:04  |g pages:1541-50 
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