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|a pubmed25n0783.xml
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|a (DE-627)NLM235146587
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|a (NLM)24495759
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Cao, Ling-feng
|e verfasserin
|4 aut
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| 245 |
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|a Profiles of innate immune receptors and cytokines in peripheral blood mononuclear cells in children with measles
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|c 2013
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 04.09.2014
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|a Date Revised 07.06.2016
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: The aim of this study was to explore the roles and clinical significance of innate immune receptors and cytokine in children with measles
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| 520 |
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|a METHOD: The children with measles hospitalized in the department of infectious diseases, Children's Hospital of Fudan University during 2009-2011 were enrolled into measles group, while the healthy children examined in well baby clinic were enrolled into control group. The mRNA expression of TLR2/3/4/7, melanoma differentiation-associated gene-5 (MDA-5), retinoic acid-inducible gene I (RIG-I), IFN-α/β and IL-10 in peripheral blood mononuclear cells were detected by real-time PCR. The protein levels of IFN-α, IFN-β and IL-10 in plasma were measured using ELISA. SPSS 13.0 software was applied to analyze the difference between two groups
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|a RESULT: Data from a total of 98 patients in measles group and 59 children in control group were collected. The mRNA expressions of TLR2, MDA-5 and RIG-I had no statistical significance between two groups (P > 0.05, respectively). The relative mRNA expressions of TLR3, TLR4, TLR7 in measles group (2.25 ± 0.74, 2.05 ± 0.72, 2.12 ± 0.29) were significantly lower than those in control group (2.09 ± 0.78, 1.90 ± 0.75, 1.87 ± 0.68) (P < 0.01; respectively). Both IFN-α and IFN-β had significantly decreased mRNA expressions in measles patients (2.41 ± 1.31, 2.47 ± 1.26) compared with those in controls (2.22 ± 0.48, 2.35 ± 0.64)(P < 0.01 respectively); however, IL-10 mRNA levels significantly increased (2.49 ± 0.58 vs. 2.62 ± 0.95) (P < 0.001). The IL-10 levels in plasma in measles group were significantly higher during the whole period of fever [<5 d group: 29.89 (25.82-38.15) ng/L and ≥ 5 d group:34.55 (28.26-38.70) ng/L] than that in control group [25.15 (24.20-27.38) ng/L] (P < 0.05 respectively)
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|a CONCLUSION: TLR3/4/7 mRNA expression was low in peripheral blood mononuclear cells of measles patients. Levels of IL-10 were significantly raised in the early stage after infection and lasted for a long time, and reduced IFN-α levels in plasma were associated with the fever durations of measles patients. These results indicated that multiple TLRs and cytokines may participate in the immune response after measles virus infection
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|a English Abstract
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Cytokines
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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7 |
|a Toll-Like Receptors
|2 NLM
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| 700 |
1 |
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|a Ye, Ying-zi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Jin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Wen-jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Xin-bao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhu, Yan-feng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shen, Jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ge, Yan-ling
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pu, Dong-bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xia, Ai-mei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Meng-hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Xiao-hong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Hui
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhonghua er ke za zhi = Chinese journal of pediatrics
|d 1960
|g 51(2013), 12 vom: 03. Dez., Seite 898-902
|w (DE-627)NLM136249191
|x 0578-1310
|7 nnns
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| 773 |
1 |
8 |
|g volume:51
|g year:2013
|g number:12
|g day:03
|g month:12
|g pages:898-902
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_11
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|a GBV_ILN_20
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|a GBV_ILN_22
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|a GBV_ILN_24
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|a GBV_ILN_31
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|a GBV_ILN_39
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|a GBV_ILN_244
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|a GBV_ILN_285
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|a GBV_ILN_294
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|a AR
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| 952 |
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|d 51
|j 2013
|e 12
|b 03
|c 12
|h 898-902
|