Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves

Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves

Copyright © 2014 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 81(2014) vom: 17. Aug., Seite 74-83
1. Verfasser: Brestic, Marian (VerfasserIn)
Weitere Verfasser: Zivcak, Marek, Olsovska, Katarina, Shao, Hong-Bo, Kalaji, Hazem M, Allakhverdiev, Suleyman I
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Barley Chlorophyll fluorescence Glutamine synthetase Non-photochemical quenching Photoinhibition Photorespiration Light-Harvesting Protein Complexes Photosystem I Protein Complex mehr... Photosystem II Protein Complex Plant Proteins Chlorophyll 1406-65-1 Carbon Dioxide 142M471B3J Glutamate-Ammonia Ligase EC 6.3.1.2
Beschreibung
Zusammenfassung:Copyright © 2014 Elsevier Masson SAS. All rights reserved.
The chloroplastic glutamine synthetase (GS, EC 6.3.1.2) activity was previously shown to be the limiting step of photorespiratory pathway. In our experiment, we examined the photosynthetic high-light responses of the GS2-mutant of barley (Hordeum vulgare L.) with reduced GS activity, in comparison to wild type (WT). The biophysical methods based on slow and fast chlorophyll fluorescence induction, P700 absorbance, and gas exchange measurements were employed. Despite the GS2 plants had high basal fluorescence (F0) and low maximum quantum yield (Fv/Fm), the CO2 assimilation rate, the PSII and PSI actual quantum yields were normal. On the other hand, in high light conditions the GS2 had much higher non-photochemical quenching (NPQ), caused both by enhanced capacity of energy-dependent quenching and disconnection of PSII antennae from reaction centers (RC). GS2 leaves also maintained the PSII redox poise (QA(-)/QA total) at very low level; probably this was reason why the observed photoinhibitory damage was not significantly above WT. The analysis of fast chlorophyll fluorescence induction uncovered in GS2 leaves substantially lower RC to antenna ratio (RC/ABS), low PSII/PSI ratio (confirmed by P700 records) as well as low PSII excitonic connectivity
Beschreibung:Date Completed 30.03.2015
Date Revised 08.04.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2014.01.002