OsPT2, a phosphate transporter, is involved in the active uptake of selenite in rice

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 201(2014), 4 vom: 04. März, Seite 1183-1191
1. Verfasser:	Zhang, Lianhe (VerfasserIn)
Weitere Verfasser:	Hu, Bin, Li, Wei, Che, Ronghui, Deng, Kun, Li, Hua, Yu, Feiyan, Ling, Hongqing, Li, Youjun, Chu, Chengcai
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't molecular mechanism phosphate transporter rice (Oryza sativa) selenite uptake selenium (Se) Phosphate Transport Proteins Phosphates Plant Proteins mehr... RNA, Messenger Symporters Carbonyl Cyanide m-Chlorophenyl Hydrazone 555-60-2 Sulfur 70FD1KFU70 Hydrogen 7YNJ3PO35Z Selenious Acid F6A27P4Q4R Selenium H6241UJ22B 2,4-Dinitrophenol Q13SKS21MN

Beschreibung
Zusammenfassung:	© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust. • Selenite is a predominant form of selenium (Se) available to plants, especially in anaerobic soils, but the molecular mechanism of selenite uptake by plants is not well understood. • ltn1, a rice mutant previously shown to have increased phosphate (Pi) uptake, was found to exhibit higher selenite uptake than the wild-type in both concentration- and time-dependent selenite uptake assays. Respiratory inhibitors significantly inhibited selenite uptake in the wildtype and the ltn1 mutant, indicating that selenite uptake was coupled with H(+) and energy-dependent. Selenite uptake was greatly enhanced under Pi-starvation conditions, suggesting that Pi transporters are involved in selenite uptake. • OsPT2, the most abundantly expressed Pi transporter in the roots, is also significantly up-regulated in ltn1 and dramatically induced by Pi starvation. OsPT2-overexpressing and knockdown plants displayed significantly increased and decreased rates of selenite uptake, respectively, suggesting that OsPT2 plays a crucial role in selenite uptake. Se content in rice grains also increased significantly in OsPT2-overexpressing plants. • These data strongly demonstrate that selenite and Pi share similar uptake mechanisms and that OsPT2 is involved in selenite uptake, which provides a potential strategy for breeding Se-enriched rice varieties
Beschreibung:	Date Completed 29.09.2014 Date Revised 03.12.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.12596