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231224s2014 xx |||||o 00| ||eng c |
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7 |
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|a 10.1021/la403525z
|2 doi
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|a pubmed24n0783.xml
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|a (DE-627)NLM234918691
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|a (NLM)24471580
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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1 |
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|a Debrassi, Aline
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Stability of (bio)functionalized porous aluminum oxide
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 21.10.2014
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|a Date Revised 11.02.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Porous aluminum oxide (PAO), a nanostructured support for, among others, culturing microorganisms, was chemically modified in order to attach biomolecules that can selectively interact with target bacteria. We present the first comprehensive study of monolayer-modified PAO using conditions that are relevant to microbial growth with a range of functional groups (carboxylic acid, α-hydroxycarboxylic acid, alkyne, alkene, phosphonic acid, and silane). Their stability was initially assessed in phosphate-buffered saline (pH 7.0) at room temperature. The most stable combination (PAO with phosphonic acids) was further studied over a range of physiological pHs (4-8) and temperatures (up to 80 °C). Varying the pH had no significant effect on the stability, but it gradually decreased with increasing temperature. The stability of phosphonic acid-modified PAO surfaces was shown to depend strongly on the other terminal group of the monolayer structure: in general, hydrophilic monolayers were less stable than hydrophobic monolayers. Finally, an alkyne-terminated PAO surface was reacted with an azide-linked mannose derivative. The resulting mannose-presenting PAO surface showed the clearly increased adherence of a mannose-binding bacterium, Lactobacillus plantarum, and also allowed for bacterial outgrowth
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Alkenes
|2 NLM
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|a Alkynes
|2 NLM
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|a Carboxylic Acids
|2 NLM
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|a Phosphoric Acids
|2 NLM
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|a Silanes
|2 NLM
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|a phosphoric acid
|2 NLM
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|a E4GA8884NN
|2 NLM
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| 650 |
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|a Aluminum Oxide
|2 NLM
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| 650 |
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|a LMI26O6933
|2 NLM
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| 700 |
1 |
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|a Ribbera, Angela
|e verfasserin
|4 aut
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1 |
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|a de Vos, Willem M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wennekes, Tom
|e verfasserin
|4 aut
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| 700 |
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|a Zuilhof, Han
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 30(2014), 5 vom: 11. Feb., Seite 1311-20
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:30
|g year:2014
|g number:5
|g day:11
|g month:02
|g pages:1311-20
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|u http://dx.doi.org/10.1021/la403525z
|3 Volltext
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