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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2013.12.020
|2 doi
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|a pubmed24n0783.xml
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|a (DE-627)NLM23489055X
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|a (NLM)24468661
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|a (PII)S0981-9428(13)00463-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Ping
|e verfasserin
|4 aut
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| 245 |
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|a Identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from Gossypium hirsutum
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.10.2014
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2014 Elsevier Masson SAS. All rights reserved.
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|a Plants have developed a variety of adaptive mechanisms to cope with stresses. A novel salt-induced gene was isolated during the screening of a NaCl-induced cDNA library of cotton seedlings. The gene was registered as accession number AY972810 in GenBank. Phylogenetic analysis suggested that the protein encoded by the gene belongs to the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases, EC 5.2.1.8). Northern blot analysis indicated that the mRNA accumulation of GhPPI was induced by salt stress. Subcellular localization revealed that GhPPI (Gossypium hirsutum peptidyl-prolyl isomerase) was localized in the nucleus. The purified recombinant GhPPI could accelerate the initial velocity of the cis-trans conversion of peptidyl-prolyl bonds of a tetrapeptide in a GhPPI concentration-dependent manner. Recombinant GhPPI also suppressed protein aggregation under denaturing conditions using Gdn-HCl (guanidine hydrochloride), suggesting an additional chaperone activity. Several amino acid residues in GhPPI were speculated to be involved in substrate binding or catalysis based on molecular modeling and docking results. The activity of the peptidyl-prolyl isomerase was affected when the relevant amino acids were mutated. Among the 11 mutants, five amino acids mutations led to the enzyme activities decreased to 30% as that of wild type, and two reduced to approximately 60%. To the best of our knowledge, this is the first report of a plant parvulin PPIase involved in the salt stress response
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Gossypium hirsutum
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|a Key amino acids
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|a PPIase
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|a Salt stress
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|a DNA, Complementary
|2 NLM
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|a Molecular Chaperones
|2 NLM
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| 650 |
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|a NIMA-Interacting Peptidylprolyl Isomerase
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Sodium Chloride
|2 NLM
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| 650 |
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|a 451W47IQ8X
|2 NLM
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| 650 |
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|a Peptidylprolyl Isomerase
|2 NLM
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| 650 |
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|a EC 5.2.1.8
|2 NLM
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| 700 |
1 |
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|a Li, Xin-Zheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cui, Hao-Ran
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Feng, Yue-guang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Xiao-Yun
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 76(2014) vom: 27. März, Seite 58-66
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:76
|g year:2014
|g day:27
|g month:03
|g pages:58-66
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|u http://dx.doi.org/10.1016/j.plaphy.2013.12.020
|3 Volltext
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|d 76
|j 2014
|b 27
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|h 58-66
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