A technique to increase protein yield in a rabbit reticulocyte lysate translation system
Rabbit reticulocyte lysate (RRL) is a mammalian cell-free system for protein production. However, one of the limitations of this system is its low protein yield. Inclusion of recombinant virus proteins and specific viral structures on target mRNA could enhance protein production in RRL. Here we show...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 56(2014), 1 vom: 07. Jan., Seite 36-9
|
|---|
| 1. Verfasser: |
Anastasina, Maria
(VerfasserIn) |
|---|
| Weitere Verfasser: |
Terenin, Ilya,
Butcher, Sarah J,
Kainov, Denis E |
|---|
| Format: | Online-Aufsatz
|
|---|
| Sprache: | English |
|---|
| Veröffentlicht: |
2014
|
|---|
| Zugriff auf das übergeordnete Werk: | BioTechniques
|
|---|
| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
EMCV IRES
cell-free translation
influenza A virus
non-structural protein
rabbit reticulocyte lysate
INS1 protein, influenza virus
RNA, Messenger
Recombinant Proteins
Viral Nonstructural Proteins |
|---|