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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1111/nph.12593
|2 doi
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|a pubmed24n0780.xml
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|a (DE-627)NLM234260572
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|a (NLM)24400899
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Kang, Heng
|e verfasserin
|4 aut
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|a A MYB coiled-coil transcription factor interacts with NSP2 and is involved in nodulation in Lotus japonicus
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.09.2014
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|a Date Revised 16.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
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|a Transcription factor complex formation is a central step in regulating gene expression. In this report, a novel MYB coiled-coil transcription factor referred to as IPN2, for Interacting Protein of NSP2, is described. The interaction between IPN2 and NSP2 was examined by protein pull-down assays and bimolecular fluorescence complementation (BiFC). Subcellular localization of proteins, gene expression and gene function were assessed in transgenic hairy roots expressing tagged recombinant proteins, promoter-reporter and RNA interference (RNAi) constructs, respectively. The GRAS domain of NSP2 and the coiled-coil domain of IPN2 were found to be responsible for the interaction between the two proteins. IPN2 had strong transcription activation activity, bound directly to the NIN gene promoter, and was localized to the nuclei of Lotus japonicus root cells. The expression of IPN2 was elevated during nodule development, coinciding with increased NSP2 gene expression during nodule organogenesis. RNAi-mediated knockdown expression of IPN2 did not affect arbuscular mycorrhizal development, but had deleterious effects on rhizobial infection and nodule formation in L. japonicus. These results demonstrate an important role of IPN2 in nodule organogenesis and place a new MYB transcription factor in the Nod signaling pathway
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Lotus japonicus
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|a MYB protein
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|a RNA interference
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|a legume
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|a nodulation
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|a transcriptional regulation
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|a DNA, Plant
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Transcription Factors
|2 NLM
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|a Glucuronidase
|2 NLM
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|a EC 3.2.1.31
|2 NLM
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1 |
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|a Chu, Xiaojie
|e verfasserin
|4 aut
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|a Wang, Chao
|e verfasserin
|4 aut
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1 |
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|a Xiao, Aifang
|e verfasserin
|4 aut
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|a Zhu, Hui
|e verfasserin
|4 aut
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|a Yuan, Songli
|e verfasserin
|4 aut
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|a Yang, Zhenzhen
|e verfasserin
|4 aut
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|a Ke, Danxia
|e verfasserin
|4 aut
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|a Xiao, Shaobo
|e verfasserin
|4 aut
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|a Hong, Zonglie
|e verfasserin
|4 aut
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|a Zhang, Zhongming
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 201(2014), 3 vom: 29. Feb., Seite 837-849
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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|g volume:201
|g year:2014
|g number:3
|g day:29
|g month:02
|g pages:837-849
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|u http://dx.doi.org/10.1111/nph.12593
|3 Volltext
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|a AR
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|d 201
|j 2014
|e 3
|b 29
|c 02
|h 837-849
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