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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.12195
|2 doi
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|a eng
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|a Low, C-F
|e verfasserin
|4 aut
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|a Experimental infection of brown-marbled grouper, Epinephelus fuscoguttatus (Forskal), with Vibrio parahaemolyticus identifies parvalbumin beta-2 subunit I, alpha-2-macroglobulin, nattectin and immunoglobulin light chain, differentially expressed in resistant grouper
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 11.05.2015
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2014 John Wiley & Sons Ltd.
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|a The mechanisms through which brown-marbled grouper accomplishes resistance to infection, particularly against Vibrios, are not yet fully understood. In this study, brown-marbled grouper fingerlings were experimentally infected with Vibrio parahaemolyticus, to identify disease resistance grouper, and the serum proteome profiles were compared between resistant and susceptible candidates, via two-dimensional gel electrophoresis (2-DE). The results showed that putative parvalbumin beta-2 subunit I, alpha-2-macroglobulin, nattectin and immunoglobulin light chain proteins were among proteins that significantly overexpressed in the resistant fish as compared to the susceptible group of fish, whereas apolipoprotein E and immunoglobulin light chain proteins were observed to be differentially overexpressed in the susceptible fish. Further analysis by peptide sequencing revealed that the immunoglobulin light chain proteins identified in the resistant and susceptible groups differed in amino acid composition. Taken together, the results demonstrated for the first time that putative parvalbumin beta-2 subunit I, alpha-2-macroglobulin, nattectin and immunoglobulin light chain are among important proteins participating to effect disease resistance mechanism in fish and were overexpressed to function collectively to resist V. parahaemolyticus infection. Most of these molecules are mediators of immune response
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 2D SDS-PAGE
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|a Vibrio parahaemolyticus
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|a fish disease
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|a grouper
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| 650 |
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4 |
|a immunoglobulin light chain
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| 650 |
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4 |
|a infection
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| 650 |
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|a Fish Proteins
|2 NLM
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| 650 |
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|a Immunoglobulin Light Chains
|2 NLM
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| 650 |
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|a Lectins, C-Type
|2 NLM
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| 650 |
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|a Parvalbumins
|2 NLM
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| 650 |
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|a alpha-Macroglobulins
|2 NLM
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| 700 |
1 |
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|a Shamsudin, M Nor
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Abdullah, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chee, H-Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aliyu-Paiko, M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 38(2015), 1 vom: 29. Jan., Seite 17-25
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
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|g volume:38
|g year:2015
|g number:1
|g day:29
|g month:01
|g pages:17-25
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|u http://dx.doi.org/10.1111/jfd.12195
|3 Volltext
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