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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2013.11.008
|2 doi
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|a pubmed25n0780.xml
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|a (DE-627)NLM234142197
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|a (NLM)24388530
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|a (PII)S0168-9452(13)00244-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Gao, Ying
|e verfasserin
|4 aut
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|a Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 03.09.2014
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
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|a Chitinases are a group of pathogenesis-related proteins. The Brassica juncea chitinase gene BjCHI1 is highly inducible by pathogenic fungal infection, suggesting that the promoter of BjCHI1 might contain specific cis-acting element responsive to fungal attack. To identify the fungus-responsive element in BjCHI1 promoter (BjC-P), a series of binary plant transformation vectors were constructed by fusing the BjC-P or its deletion-derivatives to β-glucuronidase (GUS) reporter gene. Expression of the GUS reporter gene was systematically assayed by a transient gene expression system in Nicotiana benthamiana leaves treated with fungal elicitor Hexa-N-Acetyl-Chitohexaose, as well as in transgenic Arabidopsis plants inoculated with fungus Botrytis cinerea. The histochemical and quantitative GUS assays showed that the W-box-like element (GTAGTGACTCAT) in the region (-668 to -657) was necessary for the fungus-response, although there were another five W-box-like elements in BjC-P. In addition, gain-of-function analysis demonstrated that the fragment (-409 to -337) coupled to the W-box-like element was needed for full magnitude of the fungal induction. These results revealed the existence of a novel regulation mechanism of W-box-like element involved in plant pathogenic resistance, and will benefit the potential application of BjC-P in engineering crops
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fungal elicitor
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|a Inducible promoter
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|a Transient expression
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|a W-box-like
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|a cis-Acting element
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|a Oligosaccharides
|2 NLM
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|a Plant Proteins
|2 NLM
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|a N-acetylchitohexaose
|2 NLM
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|a 38854-46-5
|2 NLM
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| 650 |
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|a Chitinases
|2 NLM
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| 650 |
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|a EC 3.2.1.14
|2 NLM
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| 650 |
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|a Glucuronidase
|2 NLM
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| 650 |
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|a EC 3.2.1.31
|2 NLM
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| 700 |
1 |
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|a Zan, Xin-Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Xue-Feng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yao, Lei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Yu-Ling
|e verfasserin
|4 aut
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| 700 |
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|a Jia, Shuang-Wei
|e verfasserin
|4 aut
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| 700 |
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|a Zhao, Kai-Jun
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 215-216(2014) vom: 07. Feb., Seite 190-8
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
1 |
8 |
|g volume:215-216
|g year:2014
|g day:07
|g month:02
|g pages:190-8
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|u http://dx.doi.org/10.1016/j.plantsci.2013.11.008
|3 Volltext
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|d 215-216
|j 2014
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|h 190-8
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