The miR172 target TOE3 represses AGAMOUS expression during Arabidopsis floral patterning

The miR172 target TOE3 represses AGAMOUS expression during Arabidopsis floral patterning

Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 215-216(2014) vom: 28. Feb., Seite 29-38
1. Verfasser: Jung, Jae-Hoon (VerfasserIn)
Weitere Verfasser: Lee, Sangmin, Yun, Ju, Lee, Minyoung, Park, Chung-Mo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't -glucuronidase AG AGAMOUS AP2 APETALA2 Arabidopsis CaMV ChIP mehr... FLOWERING LOCUS T FT Floral patterning GUSβ MS Murashige & Skoog SOC1 SPL3 SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 TARGET OF EAT 3 TOE3 cauliflower mosaic virus chromatin immunoprecipitation miR172 microRNA172 qRT-PCR quantitative real-time RT-PCR AGAMOUS Protein, Arabidopsis APETALA2 protein, Arabidopsis Arabidopsis Proteins Homeodomain Proteins MicroRNAs Nuclear Proteins Proteins TOE3 protein, Arabidopsis Transcription Factors spore photoproduct lyase EC 4.1.99.-
Beschreibung
Zusammenfassung:Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
microRNA172 (miR172) regulates phase transition and floral patterning in Arabidopsis by repressing targets that encode the APETALA2 (AP2) and AP2-like transcription factors. The miR172-mediated repression of the AP2 gene restricts AGAMOUS (AG) expression. In addition, most miR172 targets, including AP2, redundantly act as floral repressors, and the overexpression of the target genes causes delayed flowering. However, how miR172 targets other than AP2 regulate both of the developmental processes remains unclear. Here, we demonstrate that miR172-mediated repression of the TARGET OF EAT 3 (TOE3) gene is critical for floral patterning in Arabidopsis. Transgenic plants that overexpress a miR172-resistant TOE3 gene (rTOE3-ox) exhibit indeterminate flowers with numerous stamens and carpelloid organs, which is consistent with previous observations in transgenic plants that overexpress a miR172-resistant AP2 gene. TOE3 binds to the second intron of the AG gene. Accordingly, AG expression is significantly reduced in rTOE3-ox plants. TOE3 also interacts with AP2 in the nucleus. Given the major role of AP2 in floral patterning, miR172 likely regulates TOE3 in floral patterning, at least in part via AP2. In addition, a miR156 target SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 directly activates TOE3 expression, revealing a novel signaling interaction between miR156 and miR172 in floral patterning
Beschreibung:Date Completed 03.09.2014
Date Revised 09.03.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2259
DOI:10.1016/j.plantsci.2013.10.010