|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM234023309 |
| 003 |
DE-627 |
| 005 |
20250216121221.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/ert428
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0780.xml
|
| 035 |
|
|
|a (DE-627)NLM234023309
|
| 035 |
|
|
|a (NLM)24376253
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Kravchik, Michael
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Global and local perturbation of the tomato microRNA pathway by a trans-activated DICER-LIKE 1 mutant
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 25.09.2014
|
| 500 |
|
|
|a Date Revised 07.12.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a DICER-like 1 (DCL1) is a major player in microRNA (miRNA) biogenesis and accordingly, its few known loss-of-function mutants are either lethal or display arrested development. Consequently, generation of dcl1 mutants by reverse genetics and functional analysis of DCL1 in late-developing organs are challenging. Here, these challenges were resolved through the unique use of trans-activated RNA interference. Global, as well as organ-specific tomato DCL1 (SlDCL1) silencing was induced by crossing the generated responder line (OP:SlDCL1IR) with the appropriate driver line. Constitutive trans-activation knocked down SlDCL1 levels by ~95%, resulting in severe abnormalities including post-germination growth arrest accompanied by decreased miRNA and 21-nucleotide small RNA levels, but prominently elevated levels of 22-nucleotide small RNAs. The increase in the 22-nucleotide small RNAs was correlated with specific up-regulation of SlDCL2b and SlDCL2d, which are probably involved in their biogenesis. Leaf- and flower-specific OP:SlDCL1IR trans-activation inhibited blade outgrowth, induced premature bud senescence and produced pale petals, respectively, emphasizing the importance of SlDCL1-dependent small RNAs in these processes. Together, these results establish OP:SlDCL1IR as an efficient tool for analysing processes regulated by SlDCL1-mediated gene regulation in tomato
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
4 |
|a Carotenoid
|
| 650 |
|
4 |
|a DCL1
|
| 650 |
|
4 |
|a leaf
|
| 650 |
|
4 |
|a miRNA
|
| 650 |
|
4 |
|a mutant
|
| 650 |
|
4 |
|a petal
|
| 650 |
|
4 |
|a tomato.
|
| 650 |
|
7 |
|a MicroRNAs
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a RNA, Plant
|2 NLM
|
| 650 |
|
7 |
|a RNA, Small Interfering
|2 NLM
|
| 650 |
|
7 |
|a Carotenoids
|2 NLM
|
| 650 |
|
7 |
|a 36-88-4
|2 NLM
|
| 650 |
|
7 |
|a Ribonuclease III
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.26.3
|2 NLM
|
| 700 |
1 |
|
|a Sunkar, Ramanjulu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Damodharan, Subha
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Stav, Ran
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zohar, Matat
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Isaacson, Tal
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Arazi, Tzahi
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 2 vom: 24. Feb., Seite 725-39
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:65
|g year:2014
|g number:2
|g day:24
|g month:02
|g pages:725-39
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/ert428
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 65
|j 2014
|e 2
|b 24
|c 02
|h 725-39
|