pH switching that crosses over the isoelectric point (pI) can improve the entrapment of proteins within giant liposomes by enhancing protein-membrane interaction
The ability to encapsulate various molecules including proteins within giant liposomes is needed for studies on model cell membranes and artificial cells. In this report, we demonstrate how to improve the efficiency of protein entrapment with the gentle hydration (natural swelling) method, which is...
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Détails bibliographiques
Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 30(2014), 2 vom: 21. Jan., Seite 554-63
|
Auteur principal: |
Shimizu, Takahiro
(Auteur) |
Autres auteurs: |
Mori, Takaaki,
Tomita, Masahiro,
Tsumoto, Kanta |
Format: | Article en ligne
|
Langue: | English |
Publié: |
2014
|
Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids
|
Sujets: | Journal Article
Research Support, Non-U.S. Gov't
Lipid Bilayers
Liposomes
Myoglobin
Serum Albumin, Bovine
27432CM55Q
Sodium Chloride
451W47IQ8X
Muramidase
EC 3.2.1.17 |