Optimization of marine waste based-growth media for microbial lipase production using mixture design methodology
Lipase production by Staphylococcus xylosus and Rhizopus oryzae was investigated using a culture medium based on a mixture of synthetic medium and supernatants generated from tuna by-products and Ulva rigida biomass. The proportion of the three medium components was optimized using the simplex-centr...
| Veröffentlicht in: | Environmental technology. - 1993. - 34(2013), 13-16 vom: 17. Juli, Seite 2259-66 |
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| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | Environmental technology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Bacterial Proteins Culture Media Fungal Proteins Industrial Waste Lipase EC 3.1.1.3 |
| Zusammenfassung: | Lipase production by Staphylococcus xylosus and Rhizopus oryzae was investigated using a culture medium based on a mixture of synthetic medium and supernatants generated from tuna by-products and Ulva rigida biomass. The proportion of the three medium components was optimized using the simplex-centroid mixture design method (SCMD). Results indicated that the experimental data were in good agreement with predicted values, indicating that SCMD was a reliable method for determining the optimum mixture proportion of the growth medium. Maximal lipase activities of 12.5 and 23.5 IU/mL were obtained with a 50:50 (v:v) mixture of synthetic medium and tuna by-product supernatant for Staphylococcus xylosus and Rhizopus oryzae, respectively. The predicted responses from these mixture proportions were also validated experimentally |
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| Beschreibung: | Date Completed 16.01.2014 Date Revised 19.12.2013 published: Print Citation Status MEDLINE |
| ISSN: | 1479-487X |