Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed

Bibliographische Detailangaben
Veröffentlicht in:	Journal of plant physiology. - 1979. - 171(2014), 2 vom: 15. Jan., Seite 140-7
1. Verfasser:	Xin, Xia (VerfasserIn)
Weitere Verfasser:	Tian, Qian, Yin, Guangkun, Chen, Xiaoling, Zhang, Jinmei, Ng, Sophia, Lu, Xinxiong
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	Journal of plant physiology
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't APX ASC ASH ATP Artificial ageing Ascorbate–glutathione cycle BSA COX mehr... DHA DHAR GR GSH GSSG MDA MDHAR Mitochondria N-Tris (hydroxymethyl)-methyl-2-aminoethanesulfonic acid RCR ROS Reduced ascorbate SOD Soybean seed TCA TEM TES adenosine triphosphate ascorbate peroxidase ascorbic acid bovine serum albumin cytochrome c oxidase dehydroascorbate (oxidized ascorbate) dehydroascorbate reductase glutathione glutathione disulfide glutathione reductase malondialdehyde monodehydroascorbate reductase reactive oxygen species respiration control rate superoxide dismutase transmission electron microscopy tricarboxylic acid Antioxidants Reactive Oxygen Species Glutathione GAN16C9B8O Ascorbic Acid PQ6CK8PD0R

Beschreibung
Zusammenfassung:	Copyright © 2013 Elsevier GmbH. All rights reserved. The effect of artificial ageing on the relationship between mitochondrial activities and the antioxidant system was studied in soybean seeds (Glycine max L. cv. Zhongdou No. 27). Ageing seeds for 18d and 41d at 40°C reduced germination from 99% to 52% and 0%, respectively. In comparison to the control, malondialdehyde content and leachate conductivity in aged seeds increased and were associated with membrane damage. Transmission electron microscopy and Percoll density gradient centrifugation showed that aged seeds mainly contained poorly developed mitochondria in which respiration and marker enzymes activities were significantly reduced. Heavy mitochondria isolated from the interface of the 21% and 40% Percoll were analyzed. Mitochondrial antioxidant enzymes activities including superoxide dismutase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase were significantly reduced in aged seeds. A decrease in total ascorbic acid (ASC) and glutathione (GSH) content as well as the reduced/oxidized ratio of ASC and GSH in mitochondria with prolonged ageing showed that artificial ageing reduced ASC-GSH cycle activity. These results suggested an elevated reactive oxygen species (ROS) level in the aged seeds, which was confirmed by measurements of superoxide radical and hydrogen peroxide levels. We conclude that mitochondrial dysfunction in artificially aged seeds is due to retarded mitochondrial and ASC-GSH cycle activity and elevated ROS accumulation
Beschreibung:	Date Completed 02.09.2014 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1618-1328
DOI:	10.1016/j.jplph.2013.09.016