Quantitative analysis of herbivore-induced cytosolic calcium by using a Cameleon (YC 3.6) calcium sensor in Arabidopsis thaliana

Copyright © 2013 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 171(2014), 2 vom: 15. Jan., Seite 136-9
1. Verfasser: Verrillo, Francesca (VerfasserIn)
Weitere Verfasser: Occhipinti, Andrea, Kanchiswamy, Chidananda Nagamangala, Maffei, Massimo E
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana Calcium signaling Cameleon YC 3.6 Herbivory Spodoptera littoralis Calmodulin Luminescent Proteins Recombinant Fusion Proteins mehr... YCaM3.6 protein Calcium SY7Q814VUP
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245 1 0 |a Quantitative analysis of herbivore-induced cytosolic calcium by using a Cameleon (YC 3.6) calcium sensor in Arabidopsis thaliana 
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520 |a Ca(2+) is a key player in plant cell responses to biotic and abiotic stress. Owing to the central role of cytosolic Ca(2+) ([Ca(2+)]cyt) during early signaling and the need for precise determination of [Ca(2+)]cyt variations, we used a Cameleon YC 3.6 reporter protein expressed in Arabidopsis thaliana to quantify [Ca(2+)]cyt variations upon leaf mechanical damage (MD), herbivory by 3rd and 5th instar larvae of Spodoptera littoralis and S. littoralis oral secretions (OS) applied to MD. YC 3.6 allowed a clear distinction between MD and herbivory and discriminated between the two larvae instars. To our knowledge this is the first report of quantitative [Ca(2+)]cyt determination upon herbivory using a Cameleon calcium sensor 
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650 4 |a Arabidopsis thaliana 
650 4 |a Calcium signaling 
650 4 |a Cameleon YC 3.6 
650 4 |a Herbivory 
650 4 |a Spodoptera littoralis 
650 7 |a Calmodulin  |2 NLM 
650 7 |a Luminescent Proteins  |2 NLM 
650 7 |a Recombinant Fusion Proteins  |2 NLM 
650 7 |a YCaM3.6 protein  |2 NLM 
650 7 |a Calcium  |2 NLM 
650 7 |a SY7Q814VUP  |2 NLM 
700 1 |a Occhipinti, Andrea  |e verfasserin  |4 aut 
700 1 |a Kanchiswamy, Chidananda Nagamangala  |e verfasserin  |4 aut 
700 1 |a Maffei, Massimo E  |e verfasserin  |4 aut 
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