Involvement of peroxidase activity in developing somatic embryos of Medicago arborea L. Identification of an isozyme peroxidase as biochemical marker of somatic embryogenesis

Involvement of peroxidase activity in developing somatic embryos of Medicago arborea L. Identification of an isozyme peroxidase as biochemical marker of somatic embryogenesis

Copyright © 2013 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 171(2014), 2 vom: 15. Jan., Seite 78-84
1. Verfasser: Gallego, Piedad (VerfasserIn)
Weitere Verfasser: Martin, Luisa, Blazquez, Antonio, Guerra, Hilario, Villalobos, Nieves
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 4-MN 4-metoxy-α-naphtol BA Embryogenic markers IEF Isoelectric focusing Isoenzymes KIN mehr... LTQ MALDI-TOF MS medium supplemented with 2.25mM 2,4-D without cytokinins MS medium supplemented with 2mgl(−1) of 2,4-D MS medium supplemented with 9μM 2,4-D and 1μM thidiazuron MS medium supplemented with 9μM 2,4-D and 9μM benzyladenine MS medium supplemented with 9μM 2,4-D and 9μM kinetin MS/MS MSI MSI+BA MSI+KIN MSI+TDZ MSII MSMurashige and Skoog (1962) Medicago arborea L. Peroxidase activity Somatic embryogenesis TDZ benzyladenine kinetin linear ion trap matrix-assisted laser desorption/ionization time-of-flight médium tandem mass spectrometry thidiazuron Biomarkers Peroxidase EC 1.11.1.7
Beschreibung
Zusammenfassung:Copyright © 2013 Elsevier GmbH. All rights reserved.
The legume Medicago arborea L. is very interesting as regards the regeneration of marginal arid soils. The problem is that it does not have a good germinative yield. It was therefore decided to regenerate via somatic embryogenesis and find a marker of embryogenic potential. In this study, peroxidase activity was evaluated in non-embryogenic and embryogenic calli from M. arborea L. A decrease in soluble peroxidase activity is observed in its embryonic calli at the time at which the somatic embryos begin to appear. This activity is always lower in embryonic calli than in non-embryonic ones (unlike what happens in the case of wall-bound peroxidases). These results suggest that peroxidases can be considered to be enzymes involved in somatic embryogenesis in M. arborea. In addition, isozyme analyses were carried out on protein extracts using polyacrylamide gel electrophoresis. The band called P5 was detected only in embryogenic cultures at very early stages of development. This band was digested with trypsin and analyzed using linear ion trap (LTQ) mass spectrometer. In P5 isoform a peroxidase-L-ascorbate peroxidase was identified. It can be used as a marker that allows the identification of embryological potential
Beschreibung:Date Completed 02.09.2014
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1618-1328
DOI:10.1016/j.jplph.2013.09.017