Overexpression of a proton-coupled vacuolar glucose exporter impairs freezing tolerance and seed germination

Overexpression of a proton-coupled vacuolar glucose exporter impairs freezing tolerance and seed germination

© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 202(2014), 1 vom: 16. Apr., Seite 188-197
1. Verfasser: Klemens, Patrick A W (VerfasserIn)
Weitere Verfasser: Patzke, Kathrin, Trentmann, Oliver, Poschet, Gernot, Büttner, Michael, Schulz, Alexander, Marten, Irene, Hedrich, Rainer, Neuhaus, H Ekkehard
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis glucose transport stress tolerance tonoplast vacuole Arabidopsis Proteins CAB1 protein, Arabidopsis Light-Harvesting Protein Complexes mehr... Monosaccharide Transport Proteins Photosystem II Protein Complex Plant Proteins Protons RNA, Messenger Starch 9005-25-8 Glucose IY9XDZ35W2
Beschreibung
Zusammenfassung:© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
Arabidopsis vacuoles harbor, besides sugar transporter of the TMT-type, an early response to dehydration like 6 (ERDL6) protein involved in glucose export into the cytosol. However, the mode of transport of ERDL6 and the plant's feedback to overexpression of its activity on essential properties such as, for example, seed germination or freezing tolerance, remain unexplored. Using patch-clamp studies on vacuoles expressing AtERDL6 we demonstrated directly that this carrier operates as a proton-driven glucose exporter. Overexpression of BvIMP, the closest sugar beet (Beta vulgaris) homolog to AtERDL6, in Arabidopsis leads surprisingly to impaired seed germination under both conditions, sugar application and low environmental temperatures, but not under standard conditions. Upon cold treatment, BvIMP overexpressor plants accumulated lower quantities of monosaccharides than the wild-type, a response in line with the reduced frost tolerance of the transgenic Arabidopsis plants, and the fact that cold temperatures inhibits BvIMP transcription in sugar beet leaves. With these findings we show that the tight control of vacuolar sugar import and export is a key requisite for cold tolerance and seed germination of plants
Beschreibung:Date Completed 28.10.2014
Date Revised 10.04.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.12642