Overexpression of a proton-coupled vacuolar glucose exporter impairs freezing tolerance and seed germination
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
| Publié dans: | The New phytologist. - 1979. - 202(2014), 1 vom: 16. Apr., Seite 188-197 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2014
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| Accès à la collection: | The New phytologist |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Arabidopsis glucose transport stress tolerance tonoplast vacuole Arabidopsis Proteins CAB1 protein, Arabidopsis Light-Harvesting Protein Complexes plus... |
| Résumé: | © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust. Arabidopsis vacuoles harbor, besides sugar transporter of the TMT-type, an early response to dehydration like 6 (ERDL6) protein involved in glucose export into the cytosol. However, the mode of transport of ERDL6 and the plant's feedback to overexpression of its activity on essential properties such as, for example, seed germination or freezing tolerance, remain unexplored. Using patch-clamp studies on vacuoles expressing AtERDL6 we demonstrated directly that this carrier operates as a proton-driven glucose exporter. Overexpression of BvIMP, the closest sugar beet (Beta vulgaris) homolog to AtERDL6, in Arabidopsis leads surprisingly to impaired seed germination under both conditions, sugar application and low environmental temperatures, but not under standard conditions. Upon cold treatment, BvIMP overexpressor plants accumulated lower quantities of monosaccharides than the wild-type, a response in line with the reduced frost tolerance of the transgenic Arabidopsis plants, and the fact that cold temperatures inhibits BvIMP transcription in sugar beet leaves. With these findings we show that the tight control of vacuolar sugar import and export is a key requisite for cold tolerance and seed germination of plants |
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| Description: | Date Completed 28.10.2014 Date Revised 10.04.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.12642 |