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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ert412
|2 doi
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|a pubmed24n0778.xml
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|a (DE-627)NLM233522123
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|a (NLM)24323506
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Shih, Ching-Fang
|e verfasserin
|4 aut
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| 245 |
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|a The NAC-like gene ANTHER INDEHISCENCE FACTOR acts as a repressor that controls anther dehiscence by regulating genes in the jasmonate biosynthesis pathway in Arabidopsis
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 25.09.2014
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|a Date Revised 21.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a ANTHER INDEHISCENCE FACTOR (AIF), a NAC-like gene, was identified in Arabidopsis. In AIF:GUS flowers, β-glucuronidase (GUS) activity was detected in the anther, the upper parts of the filaments, and in the pollen of stage 7-9 young flower buds; GUS activity was reduced in mature flowers. Yellow fluorescent protein (YFP)+AIF-C fusion proteins, which lacked a transmembrane domain, accumulated in the nuclei of the Arabidopsis cells, whereas the YFP+AIF fusion proteins accumulated in the membrane and were absent in the nuclei. Further detection of a cleaved AIF protein in flowers revealed that AIF needs to be processed and released from the endoplasmic reticulum in order to function. The ectopic expression of AIF-C caused a male-sterile phenotype with indehiscent anthers throughout flower development in Arabidopsis. The presence of a repressor domain in AIF and the similar phenotype of indehiscent anthers in AIF-C+SRDX plants suggest that AIF acts as a repressor. The defect in anther dehiscence was due to the down-regulation of genes that participate in jasmonic acid (JA) biosynthesis, such as DAD1/AOS/AOC3/OPR3/OPCL1. The external application of JA rescued the anther indehiscence in AIF-C and AIF-C+SRDX flowers. In AIF-C+VP16 plants, which are transgenic dominant-negative mutants in which AIF is converted to a potent activator via fusion to a VP16-AD motif, the anther dehiscence was promoted, and the expression of DAD1/AOS/AOC3/OPR3/OPCL1 was up-regulated. Furthermore, the suppression of AIF through an antisense strategy resulted in a mutant phenotype similar to that observed in the AIF-C+VP16 flowers. The present data suggest a role for AIF in controlling anther dehiscence by suppressing the expression of JA biosynthesis genes in Arabidopsis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a ANTHER INDEHISCENCE FACTOR
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|a Anther dehiscence
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|a NAC-like gene
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|a jasmonate signalling
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|a repressor.
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|a Arabidopsis Proteins
|2 NLM
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|a Cyclopentanes
|2 NLM
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|a DNA, Complementary
|2 NLM
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|a Oxylipins
|2 NLM
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| 650 |
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|a Repressor Proteins
|2 NLM
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|a jasmonic acid
|2 NLM
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|a 6RI5N05OWW
|2 NLM
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|a Glucuronidase
|2 NLM
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|a EC 3.2.1.31
|2 NLM
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|a Hsu, Wei-Han
|e verfasserin
|4 aut
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|a Peng, Yan-Jhu
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Chang-Hsien
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 65(2014), 2 vom: 01. Feb., Seite 621-39
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:65
|g year:2014
|g number:2
|g day:01
|g month:02
|g pages:621-39
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|u http://dx.doi.org/10.1093/jxb/ert412
|3 Volltext
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|d 65
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|h 621-39
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