Low temperature inhibits pollen tube growth by disruption of both tip-localized reactive oxygen species and endocytosis in Pyrus bretschneideri Rehd

Low temperature inhibits pollen tube growth by disruption of both tip-localized reactive oxygen species and endocytosis in Pyrus bretschneideri Rehd

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 74(2014) vom: 03. Jan., Seite 255-62
1. Verfasser: Gao, Yong-Bin (VerfasserIn)
Weitere Verfasser: Wang, Chun-Lei, Wu, Ju-You, Zhou, Hong-Sheng, Jiang, Xue-Ting, Wu, Jun, Zhang, Shao-Ling
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide DPI Endocytosis FM4-64 LT LT4 Low temperature Mitochondria mehr... Mn-5,10,15,20-tetrakis(1-methyl-4-pyridyl) 21H,23H-porphin N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide NADPH oxidase NBT NOX Nitroblue tetrazolium PM Pyrus bretschneideri Rehd. ROS Reactive oxygen species TMPP XTT diphenylene iodonium chloride low temperature low temperature of 4 °C plasma membrane reactive oxygen species Reactive Oxygen Species Leukotriene C4 2CU6TT9V48 Hydrogen Peroxide BBX060AN9V Nitric Oxide Synthase EC 1.14.13.39
Beschreibung
Zusammenfassung:Copyright © 2013 Elsevier Masson SAS. All rights reserved.
Low temperature (LT) negatively affects fertilization processes of flowering plants. Pollen tube growth is generally inhibited under LT stress; however, the mechanism(s) underlying this inhibition remain(s) largely unknown. Pollen tubes are tip-growing and the presence of tip-localized reactive oxygen species (ROS) is necessary for cellular functioning. Disruption of tip-localized ROS was observed in pear pollen tubes in vitro under low temperature of 4 °C (LT4). Diphenylene iodonium chloride, an NADPH oxidase (NOX) inhibitor, suppressed hydrogen peroxide formation in the cell walls of the subapical region in pear pollen tubes. Under LT4 stress, ROS disruption in pear pollen tubes mainly resulted from decreased NOX activity in the plasma membrane, indicating that NOX was the main source of ROS in this process. Moreover, LT4 remarkably decreased mitochondrial oxygen consumption and intracellular ATP production. The endocytosis, an energy-dependent process, disruption in pear pollen tubes under LT4 may be mediated by mitochondrial metabolic dysfunctions. Our data showed ROS and endocytosis events in pear pollen tubes responding to LT4 stress
Beschreibung:Date Completed 22.09.2014
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2013.11.018