Disruption of the microtubule network alters cellulose deposition and causes major changes in pectin distribution in the cell wall of the green alga, Penium margaritaceum

Application of the dintroaniline compound, oryzalin, which inhibits microtubule formation, to the unicellular green alga Penium margaritaceum caused major perturbations to its cell morphology, such as swelling at the wall expansion zone in the central isthmus region. Cell wall structure was also not...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 65(2014), 2 vom: 27. Feb., Seite 465-79
1. Verfasser: Domozych, David S (VerfasserIn)
Weitere Verfasser: Sørensen, Iben, Sacks, Carly, Brechka, Hannah, Andreas, Amanda, Fangel, Jonatan U, Rose, Jocelyn K C, Willats, William G T, Popper, Zoë A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Cellulose Penium. homogalacturonan microtubule oryzalin pectin Antibodies, Monoclonal Dinitrobenzenes mehr... Polysaccharides Sulfanilamides rhamnogalacturonan I 662E385DWH Pectins 89NA02M4RX 9004-34-6 Glycoside Hydrolases EC 3.2.1.- glycanase polygalacturonic acid VV3XD4CL04
Beschreibung
Zusammenfassung:Application of the dintroaniline compound, oryzalin, which inhibits microtubule formation, to the unicellular green alga Penium margaritaceum caused major perturbations to its cell morphology, such as swelling at the wall expansion zone in the central isthmus region. Cell wall structure was also notably altered, including a thinning of the inner cellulosic wall layer and a major disruption of the homogalacturonan (HG)-rich outer wall layer lattice. Polysaccharide microarray analysis indicated that the oryzalin treatment resulted in an increase in HG abundance in treated cells but a decrease in other cell wall components, specifically the pectin rhamnogalacturonan I (RG-I) and arabinogalactan proteins (AGPs). The ring of microtubules that characterizes the cortical area of the cell isthmus zone was significantly disrupted by oryzalin, as was the extensive peripheral network of actin microfilaments. It is proposed that the disruption of the microtubule network altered cellulose production, the main load-bearing component of the cell wall, which in turn affected the incorporation of HG in the two outer wall layers, suggesting coordinated mechanisms of wall polymer deposition
Beschreibung:Date Completed 25.09.2014
Date Revised 21.10.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/ert390