Potassium nutrition of ectomycorrhizal Pinus pinaster : overexpression of the Hebeloma cylindrosporum HcTrk1 transporter affects the translocation of both K(+) and phosphorus in the host plant
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
| Veröffentlicht in: | The New phytologist. - 1979. - 201(2014), 3 vom: 27. Feb., Seite 951-960 |
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| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Hebeloma cylindrosporum ectomycorrhizal symbiosis in situ hybridization overexpression phosphate transporter HcPT1.1 plant potassium nutrition potassium-sodium transporter HcTrk1 mehr... |
| Zusammenfassung: | © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust. Mycorrhizal associations are known to improve the hydro-mineral nutrition of their host plants. However, the importance of mycorrhizal symbiosis for plant potassium nutrition has so far been poorly studied. We therefore investigated the impact of the ectomycorrhizal fungus Hebeloma cylindrosporum on the potassium nutrition of Pinus pinaster and examined the involvement of the fungal potassium transporter HcTrk1. HcTrk1 transcripts and proteins were localized in ectomycorrhizas using in situ hybridization and EGFP translational fusion constructs. Importantly, an overexpression strategy was performed on a H. cylindrosporum endogenous gene in order to dissect the role of this transporter. The potassium nutrition of mycorrhizal pine plants was significantly improved under potassium-limiting conditions. Fungal strains overexpressing HcTrk1 reduced the translocation of potassium and phosphorus from the roots to the shoots of inoculated plants in mycorrhizal experiments. Furthermore, expression of HcTrk1 and the phosphate transporter HcPT1.1 were reciprocally linked to the external inorganic phosphate and potassium availability. The development of these approaches provides a deeper insight into the role of ectomycorrhizal symbiosis on host plant K(+) nutrition and in particular, the K(+) transporter HcTrk1. The work augments our knowledge of the link between potassium and phosphorus nutrition via the mycorrhizal pathway |
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| Beschreibung: | Date Completed 01.09.2014 Date Revised 16.04.2021 published: Print-Electronic CommentIn: New Phytol. 2014 Feb;201(3):707-9. - PMID 24400896 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.12603 |