Observing the mushroom-to-brush transition for kinesin proteins
The height of polymers grafted to a surface is predicted to be constant at low densities ("mushroom" regime) and increase with the third root of the polymer surface density at high densities ("brush" regime). This mushroom-to-brush transition is explored with kinesin-1 proteins a...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 29(2013), 49 vom: 10. Dez., Seite 15142-5 |
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| Weitere Verfasser: | , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, Non-P.H.S. Kinesins EC 3.6.4.4 |
| Zusammenfassung: | The height of polymers grafted to a surface is predicted to be constant at low densities ("mushroom" regime) and increase with the third root of the polymer surface density at high densities ("brush" regime). This mushroom-to-brush transition is explored with kinesin-1 proteins adhered to a surface at controlled densities. The kinesin height is measured by attaching fluorescently labeled microtubules to the kinesins and determining their elevation using fluorescence interference contrast microscopy. Our measurements are consistent with a mushroom regime and a brush regime and a transition near the theoretically predicted density. The mushroom-to-brush transition may play a role in protein behavior in crowded cellular environments and may be exploited as a signal in intracellular regulation and mechanotransduction |
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| Beschreibung: | Date Completed 24.07.2014 Date Revised 03.12.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la4030712 |